Vectors for animal cells and use thereof

Details Vectors for animal cells and use thereof Vectors for animal cells and use thereof

2007-03-27

2007-03-27

Priebe, Scott D. (Department: 1633)

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

Introduction of a polynucleotide molecule into or...

C435S325000, C435S358000

Reexamination Certificate

active

11284905

ABSTRACT:
Protein synthesis inhibitor resistance genes (typified by a cycloheximide resistance gene) are capable of imparting resistance to a protein synthesis inhibitor (typified by cycloheximide) to animal cells sensitive to the inhibitor. The genes have a sequence mutated by substitution in a gene encoding a ribosome-constituting protein derived from an animal. The genes may be placed in recombinant vectors, including expression vectors containing the gene together with a foreign protein structural gene.

REFERENCES:
patent: WO 93/02201 (1993-02-01), None
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Jahn et al., “Sequence of the macronuclear DNA encoding large subunit ribosomal protein 29 (L29) inEuplotes crassusand cycloheximide sensitivity”, Gene, 151 (1994), 231-235.
Kondo, K. et al., A transformation system for the yeastCandida utilis: use of a modified endogenous ribosomal protein gene as a drugresistant marker and ribosomal DNA as an integration target for vector DNA. J. Vacteriol., vol. 177, No. 24, pp. 7171-7177 (1995).
Mutoh et al., “Inducible Expression of a Gene Encoding an L41 Ribosomal Protein Responsible for the Cycloheximide-Resistant Phenotype in the YeastCandida maltosa,” 1995, J. Bacteriol., vol. 177, pp. 5383-5386.
Rhoads et al., “Emetine Resistance of Chinese Hamster Cells: Structures of Wild-Type and Mutant Ribosomal Protein S14 mRNAs,” 1985, Mol. Cell. Biol., vol. 5, pp. 1655-1659.
Stevens et al., “Cycloheximide resistance conferred by novel mutations in ribosomal protein L41 ofChlamydomonas reinhardtii”, Mol. Gen. Genet. (2001), 264: 790-795.

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