Vectors derived from South African Arbovirus No. 86

Details Vectors derived from South African Arbovirus No. 86 Vectors derived from South African Arbovirus No. 86

2006-01-03

2006-01-03

Mosher, Mary E. (Department: 1648)

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Recombinant virus encoding one or more heterologous proteins...

C424S093200, C536S023720, C435S235100, C435S236000, C435S320100, C435S325000, C435S471000, C435S472000

Reexamination Certificate

active

06982087

ABSTRACT:
Provided herein are alphavirus vectors derived from South African Arbovirus No. 86 (S.A.AR86) comprising attenuating mutations and methods of making the same. Also provided are improved viral vectors and helper constructs comprising a S.A.AR86 capsid enhancer sequence. The present invention also provides S.A.AR86 replicon and helper constructs comprising an alphavirus capsid enhancer sequence. Further provided are methods of administering an alphavirus vector comprising a heterologous nucleotide sequence (preferably encoding an immunogen or a therapeutic polypeptide) according to the invention to a cell or subject. In preferred embodiments, the alphavirus vector delivers the heterologous nucleotide sequence to the cells of the bone, bone marrow, and/or bone-associated connective tissue.

REFERENCES:
patent: 4650764 (1987-03-01), Temin et al.
patent: 5091309 (1992-02-01), Schlesinger et al.
patent: 5185440 (1993-02-01), Davis et al.
patent: 5217879 (1993-06-01), Huang et al.
patent: 5505947 (1996-04-01), Johnston et al.
patent: 5639650 (1997-06-01), Johnston et al.
patent: 5643576 (1997-07-01), Johnston et al.
patent: 5739026 (1998-04-01), Garoff et al.
patent: 5766602 (1998-06-01), Xiong et al.
patent: 5789245 (1998-08-01), Dubensky et al.
patent: 5792462 (1998-08-01), Johnston et al.
patent: 5811407 (1998-09-01), Johnston et al.
patent: 5814482 (1998-09-01), Dubensky, Jr. et al.
patent: 5843723 (1998-12-01), Dubensky, Jr. et al.
patent: 6008035 (1999-12-01), Johnston et al.
patent: 6015694 (2000-01-01), Dubensky et al.
patent: 6156304 (2000-12-01), Glorioso et al.
patent: 6156558 (2000-12-01), Johnston et al.
patent: 6190666 (2001-02-01), Garoff et al.
patent: 6224879 (2001-05-01), Sjöberg et al.
patent: 6242259 (2001-06-01), Polo et al.
patent: 6521235 (2003-02-01), Johnston et al.
patent: 6531135 (2003-03-01), Johnston et al.
patent: 6541010 (2003-04-01), Johnston et al.
patent: 6583121 (2003-06-01), Johnston et al.
patent: 2001/0016199 (2001-08-01), Johnston et al.
patent: WO92/10578 (1992-06-01), None
patent: WO/95/07994 (1995-03-01), None
patent: WO95/27044 (1995-10-01), None
patent: WO95/31565 (1995-11-01), None
patent: WO96/17072 (1996-06-01), None
patent: WO96/37220 (1996-11-01), None
patent: WO96/37616 (1996-11-01), None
patent: WO97/38087 (1997-10-01), None
patent: 98/36779 (1998-08-01), None
patent: WO99/51263 (1999-10-01), None
patent: WO00/39318 (2000-07-01), None
patent: WO01/16343 (2001-03-01), None
Li et al PNAS 93:11658-11663, 1996.
Frolov et al., “Translation of Sindbis Virus mRNA: Analysis of Sequences Downstream of the Initiationg AUG Codon That Enhance Translation,”Journal of Virology70(2): 1182-1190 (1996).
International Search Report for the corresponding International Application No. PCT/US01/27644.
“Sindbis Virus (HRSP and Wild-Type Strains), Complete Genome,”EMBL Database, Accession Nos. J02363, 02364, J02366, J02367, and V00073,(Jul. 3, 1991).
Bredenbeek et al,Sindbis Virus Expression Vectors: Packaging of RNA Replicons by Using Defective Helper RNAs, Journal of Virology67 No. 11, pp. 6439-6446 (1993).
Bulychyov et al., “Disease Course in Guinea Pigs After Aerogenous Infection with Venezuelan Equine Encaphalomyelits Virs,” (1995).
Corsini et al.:Efficiency of Transduction by Recombinant Sindbis Replicon Virus Varies Among Cell Lines, Including Mosquito Cells and Rat Sensory Neurons,BioTechniques, 21:3 (492-497), Sep. 1996.
Davis et al,A Genetically Engineered Live Virus Vaccine for Venezuelan Equine Encephalitis; J Cell BiochemistrySupplement O No. 17 Part D, issued 1993, Abstract N404.
Davis et al,Attenuated Mutants of Venezuelan Equine Encephalitis Virus Containing Lethal Mutations in the PE2 Cleavage Signal Combined with a Second-Site Suppressor Mutation in E1, Virology212:102-110 (1995).
Davis et al.,Attenuating Mutations in the E2 Glycoprotein Gene of Venezuelan Equine Encephalitis Virus: Construction of Single and Multiple Mutants in a Full-Length cDNA Clone, Virology183 20-31 (1991).
Davis, N.L., “A molecular genetic approach to the study of Venezuelan equine encephalitis virus pathogenesis.”Archives of Virology.(1994), 99-109.
Dubensky, Jr. et al. “Sindbis Virus DNA-Based Expression Vectors: Utility for in Vitro and in Vivo Gene Transfer,”Journal of Virology,70:1 508-519 (Jan. 1996).
Frolov et al., “Translation of Sindbis Virus mRNA: Analysis of Sequences Downstream of the Initiating AUG Codon that Enhances Translation,”Journal of Virology,70:2 1182-1190 (Feb. 1996).
Frolov et al.,Alphavirus-based expression vectors: Stategies and applications, Proc. Natl. Acad. Sci. USA,vol. 93, pp. 11371-11377 (1996).
Frolov et al., “Translation of Sindbis Virus in RNA: Effects of Sequences Downstream of the Initiating Codon,”Journal of Virology,68 8111-end (1994).
Frolova et al., “Packaging Signals in Alphaviruses,”Journal of Virology,71 248-258 (Jan. 1997).
Geigenmuller-Gnirke et al. “Complementation Between Sindbis Viral RNAs Produces Infectious Particles with a Bipartite Genome.”Proceedings of the National Academy of Sciences of the United States of America,vol. 88, Issue 8 (Apr. 15, 1991), 3253-3257.
Grieder et al.,Specific Restrictions in the Progression of Venezuelan Equine Encephalitis Virus-Induced Disease Resulting from Single AminoAcid Changes in the Glycoproteins, Virology,206, pp. 994-1006 (1995).
Hahn et al., “Infectious Sindbis Virus Transient Expression Vectors for Studying Antigen Processing and Presentation,”Proc. Natl. Acad. Sci. USA,89 2679-2683 (1992).
Heise et al., “A Single Amino Acid Change in nsP1 Attenuates Neuroviolence of the Sindbis-Group Alphavirus S.S.AR86,”Journal of Virology,74:9 (May 2000), 4207-4213.
Heise et al., “Adult Mouse Neurovirulence Determinants Within the Nonstructural Genes of the Sindbis-Group Alphavirus S.A.AR86,”American Society of Virology Meeting, Colorado,Oral Presentation (Jul. 11, 2000).
Heise et al., “Sindbis-Group Alphavirus Replication in Periosteum and Endosteum of Long Bones in Adult Mice,”Journal of Virology,74:19 9294-9299 (Oct. 2000—published on-line Sep. 11, 2000).
Heise et al., “The Role of Viral Nonstructural Genes in Neuroviruience of the Sindbis-Group Virus, S.A.AR86,”Keystone Symposia, Taos, New Mexico,306 (Feb. 2000).
Hodgson et al., “Expression of Venezuelan Equine Encephalitis Virus Proteins by Recombinant Baculoviruses,”The American Journal of Tropical Medicine andHygiene, 49:3. 195-196 (1993).
Kinney, Richard, “Attenuation of Venzuelan Equine Ecephalitis Virus Strain TC-83 Is Encoded by the 5′-Noncoding Region and the E2 Envelope Glycoprotein.”Journal of Virology.vol. 67, No. 3, (Mar. 1993), 1269-1277.
Lemm et al.,Polypeptide requirements for assembly of functional Sindbis virus replication complexes: a model for the temporal regulation of minus- and plus-strand RNA synthesis, The EmBO Journal,vol. 13, No. 12, pp. 2925-2934 (1994).
Liljeström et al.,A New Generation of Animal Cell Expression Vectors Based on the Semliki Forest Virus Replicon, Bio/Technology,vol. 9, Dec. 1991, pp. 1356-1361.
Liljeström, Peter, “Alphavirus expression systems.”Current Opinion in Biotechnology.vol. 5, No. 5, (Oct. 1994), 495-500.
Liljeström, Peter, “Alphavirus Vectors for Gene Delivery,”OECD Documents, Gene Delivery Systems,109-118 (1996).
London et al., “Infectious Enveloped RNA Virus Antigenic Chimeras,”Proc. Natl. Acad. Sci. USA,89 207-211 (1992).
Lvov et al., “‘Karshi’ Virus, a New Flavivirus (Togaviridae) Isolated from Ornithodoros Papillipes (Birula, 1895) Ticks in Uzbek S.S.R.,”Archives of Virology,50 29-36 (1976).
McKnight et al., “Deduced Consensus Sequences of Sindbis Virus Strain AR339: Mutations Contained in Laboratory Strains which Affect Cell Culture and in Vivo Phenotypes,”Journal o f Virology,70(3) 1981-1989 (1996).
Morgenstein et al,Advanced mammalian g

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