Vectors containing multiple promoters in the same orientation

Details Vectors containing multiple promoters in the same orientation Vectors containing multiple promoters in the same orientation

1993-07-29

1996-08-20

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

4353201, 536 241, C12N 1511, C12N 1570, C12P 1934

Patent

active

055478620

ABSTRACT:
Disclosed are novel DNA segments, vectors and plasmids containing multiple promoters for use with various polymerases in order to transcribe cloned DNA into RNA. A preferred vector, termed pTRIPLEscript.TM., is described which contains the SP6, T7, and T3 phage promoters in the same orientation and on the same side of a multiple cloning site. This vector efficiently synthesizes in vitro transcripts from all three promoters under conditions of both limiting and saturating nucleotide concentrations. This vector also promotes transcription without crosstalk, i.e., without nonspecific initiation at inappropriate promoters.

REFERENCES:
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