Vectors comprising CpG islands without position effect...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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C536S023100, C514S04400A, C424S093100

Reexamination Certificate

active

07812148

ABSTRACT:
Polynucleotides and vectors comprising an expressible nucleic acid flanked by a 5′ extended methylation-free CpG island and a 3′ selectable marker element are disclosed. Such polynucleotides and vectors provide a means for obtaining high levels of expression of the flanked expressible nucleic acid. Preferred embodiments include combinations of 5′ extended methylation-free CpG islands and 3′ antibiotic resistance genes.

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