Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
2008-06-17
2008-06-17
Ramirez, Delia M. (Department: 1652)
Chemistry: molecular biology and microbiology
Vector, per se
C435S252300, C435S325000, C435S252330, C435S189000, C435S025000, C435S069100, C435S194000, C435S227000, C536S023200, C530S350000
Reexamination Certificate
active
07387893
ABSTRACT:
Novel DNA constructs and host cells comprising the same are disclosed. DNA constructs comprise a transcription unit (e.g. operon) comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin or a uridine kinase gene and/or a dCTP deaminase gene. In preferred embodiments the constructs comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin further comprise DNA sequences encoding for thymidylate synthase and/or transcription units comprising sequences encoding for uridine kinase preferably together with dCTP deaminase. In particularly preferred embodiments, the host cells comprise constructs having all of the above characteristics wherein the host cell displays repressed or no uracil DNA glycosylase activity. This may be achieved by removal of the host cell ung gene. Use of host cells in the manufacture of pyrimidine deoxyribonucleotides e.g. thymidine is also disclosed.
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Anderson David Martin
Liu Lin
Podkovyrov Sergey
Wang Baomin
Conger Michael M.
Ramirez Delia M.
SmithKline Beecham Corporation
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