Vectors and transformed host cells for recombinant protein produ

Details Vectors and transformed host cells for recombinant protein produ Vectors and transformed host cells for recombinant protein produ

1994-07-21

1997-08-05

Degen, Nancy

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4353201, 536 241, C12P 2102, C12N 1570, C12N 1573, C07H 2104

Patent

active

056541694

ABSTRACT:
The invention relates to an expression vector, the nucleic acid sequence of which comprises a promoter that is capable of controlling, when the vector is in a bacterial host and the temperature is lowered to below about 20.degree. C., the production of recombinant peptide or protein encoded by a gene contained within the vector. The invention also relates to host cells containing the vectors and to a method of producing a recombinant protein.

REFERENCES:
Giladi, et al., J. Mol. Biol. 224 937-948 (1992) Supercoiling, Integration Host Factor, and a Dual Promoter System . . . .
Giladi, et al. Proc. Natl. Acad. Sci. U.S.A. 92 2184-2188 (1995) Enhanced Activity of the Bacteriophage .lambda. P.sub.L Promoter . . . .
Lee, et al. Molecular Microbiology 11 833-839 (1994) Family of the Major Cold-Shock Protein, CSPA (C57.4), of Escherichia . . . .
Shirano, et al. FEBS Letters 271 128-130 (1990) Low Temperature Cultivation of Escherichia coli Carrying . . . .
Tanabe et al. J. Bacteriol. 174 3867-3873 (1993) Identification of the Promoter Region of the Escherichia coli Major Cold Shock . . . .
Kulakauskas et al., J. Bacteriol. 173 2633-2638 (1991) Efficient Introduction of Cloned Mutant Alleles Into . . . .
Horn et al. J. Biol. Chem. 256 2003-2009 (1981) The Leftward Promoter of Bacteriophage .lambda..

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