Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-07-20
1998-03-10
Degen, Nancy
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 536 241, C12P 2102, C12N 1520, C12N 1573, C07H 2104
Patent
active
057260397
ABSTRACT:
The invention relates to an expression vector, the nucleic acid sequence of which comprises a promoter that is capable of controlling, when the vector is in a bacterial host and the temperature is lowered to below about 20.degree. C., the production of recombinant peptide or protein encoded by a gene contained within the vector. The invention also relates to host cells containing the vectors and to a method of producing a recombinant protein.
REFERENCES:
Giladi et al. Supercoiling, integration host factor, and a dual promoter tem, participate in the control of the bacteriophage lambda pL promoter J. Mol. Biol. vol. 224 937-948, (1992).
Giladi et al. Enhanced activity of the bacteriophage lambda pL promoter at low temperature Proc. Natl. Acad. Sci. U.S.A. vol. 92 2184-2188, (1995).
Lee et al. Family of the major cold-shock protein, CspA )CS7.4), of Escherichia coli, whose members show a high sequence similarity withthe eukaryotic y-box binding proteins Mol. Mocrobiol. vol. 11 833-839, (1994).
Shirano et al. Low temperature cultivation of Escherichia coli carrying a rice lipoxygenase L-2 cDNA produces a soluble and active enzyme at a high level FEBS Lett. vol. 271 128-130, (1990).
Tanabe et al. Identification of the promoter region of the Escherichia coli major cold shock gene, cspA J. Bacteriol. vol. 174 3867-3873, (1992).
Kulakauskas et al. Efficient introduction of cloned mutant alleles into the Escherichia coli chromosome J. Bacteriol. vol. 173 2633-2638, (1991).
Horne et al. The leftward promoter of bacteriophage lambda J. Biol. Chem. vol. 256 2003-2009, (1981).
Azar Idit
Giladi Hilla
Goldenberg Daniel
Kobi Simi
Oppenheim Amos B.
Brusca John S.
Degen Nancy
Yissum Research Development Co. of The Hebrew University of Jeru
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