Vectors and prokaryotes which autocatalytically delete antibioti

Details Vectors and prokaryotes which autocatalytically delete antibioti Vectors and prokaryotes which autocatalytically delete antibioti

1995-04-20

1998-04-07

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4352523, 4353201, 935 65, C12N 1500, C12N 120, C12N 1509, C12N 1563

Patent

active

057363676

ABSTRACT:
A vector and a prokaryote transformed therewith which includes nucleic acid sequences which make possible the autocatalytic deletion of nucleotide sequences encoding an antibiotic resistance phenotype. The prokaryote can be a bacterium, and in particular a mycobacterium. Such transformed mycobacteria may be employed in vaccines, thereby eliminating the attendant risk of vaccines including antibiotic resistance markers.

REFERENCES:
patent: 5478745 (1995-12-01), Samulski et al.
patent: 5512452 (1996-04-01), Mekalanos et al.
Haun et al., "In vivo deletion of antibiotic resistance markers from Mycobacterium smegmatis and M. bovis BCG using .gamma..delta. resolvase", 94th General Meeting of the American Society for Microbiology, May 23-27, 1994; Biol. Abstr./RRM 46(8): MT-133.
Stover et al., "New use of BCG for recombinant vaccines", Nature 351:456-460, Jun. 1991.
Camilli et al., "Use of genetic recombination as a reporter of gene expression", Proc. Nat. Acad. Sci. USA 91: 2634-2638, Mar. 1994.
Dale et al., "Gene transfer with subsequent removla of the selection gene from the host genome", Proc. Nat. Acad. Sci. USA 88: 10558-10562, Dec. 1991.

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