Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1996-08-01
1998-09-01
Wax, Robert
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4351721, 4352523, 43525233, 43525411, 43525421, 4353201, 536 231, C12N 1500, C12N 114, C12N 1563
Patent
active
058010327
ABSTRACT:
A process for producing high purity 6,12-dideoxyerythromycin A using recombinant DNA technology is disclosed. The erythromycin producing strain, Saccharopolyspora erythraea, lacking the erythromycin C-12 and C-6 hydroxylases produces a mixture of 6,12-dideoxyerythromycin A and the precursor molecule, 6-deoxyerythromycin D. To achieve conversion of the precursor to the final product, a second copy of eryG is inserted into a non-essential region of the Sac. erythraea chromosome resulting in high purity 6,12-dideoxyerythromycin A.
REFERENCES:
patent: 5141926 (1992-08-01), Weber et al.
Weber et al. "An erythromycin derivatives produces by targeted gene disruption in Saccharopolyspora erythraea" Science 252, 114-117, Apr. 5, 1991.
Janssen et al. "Complex and unusual patterns of transcriptional initiation . . . " Development in Industrial Microbiology (J. of Indust. Micrbiol., Supp. 3), vol. 29, 89-96, 1988.
Doull et al. "A criptic plasmid in the chloramphenicol-producing actinomycete, Streptomyces Phaeochromogenes" FEMS Microbiol. Lett. 16, 349-352, 1983.
Haydock et al. Cloning and sequence analysis of gene involved in erythromycin biosynthesis . . . Mol. Gen. Genet. 230, 120-128, 1991.
Paulus et al. "Mutation and cloning of eryG, The structural gene for erythromycin O-methyltransferase from . . . " J. Bacteriol. 172, 2541-2546, May, 1990.
Biotechnology Letters, vol. 15, No. 2 (Feb. 1993), pp. 105-110, F. Hanel et al., "Stimulation of Erythromycin A Yield by Integration of a Chromosomal DNA Fragment Including the eryC1 Gene into the Chromosome of Saccharopolyspora Erythraea".
Gene, vol. 75 (1989), pp. 235-241, J. M. Weber, et al., "Identification of a Gene Required for the Terminal Step in Erythromycin A Biosynthesis in Saccharopolyspora Erythraea (streptomyces erythreus). "
Journal Of Bacteriology, vol. 175, No. 1, (Jan. 1993), pp. 182-189, D. Stassi, et al., Identification of a Saccharopolyspora erythraea Gene Required for the Final Hydroxylation Step in Erythromycin Biosynthesis.
Biotechnology, vol. 8, No. 2, (Feb. 1990), pp. 115-121, K. F. Chater, "The Improving Prospects for Yield Increase by Genetic Engineering in Antibiotic-Producing Streptomycetes".
S. Donadio, et al., "Recent Developments in the Genetics of Erythromycin Formation" in: Industrial Microorganisms: Basic and Applied Molecular Genetics, pp. 257-265, edited by R. H. Baltz, G. D. Hegemann, P. L. Skatrud.
Molecular And General Genetics, vol. 203, (1986), pp. 79-88, D. J. Lydiate, et al., "A 2.6 kb DNA Sequence of Streptomyces coelicolor A3(2) which functions as a Transposable Element".
Maine Gregory T.
Post David A.
Satter Mark T.
Stassi Diane L.
Abbott Laboratories
Casuto Dianne
Danckers Andreas M.
Nashed Nashaat T.
Wax Robert
LandOfFree
Vectors and process for producing high purity 6,12-dideoxyerythr does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Vectors and process for producing high purity 6,12-dideoxyerythr, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Vectors and process for producing high purity 6,12-dideoxyerythr will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-269408