Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Patent
1999-05-07
2000-04-18
Brusca, John S.
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
4353201, 435325, 536 231, C12N 1587, C12N 500, C07H 2104
Patent
active
060514302
ABSTRACT:
The present invention provides novel elements for improving genetic engineering techniques for producing recombinant nucleic acid molecules and/or recombinant cells. The novel elements are capable of integrating desired nucleic acid material into other nucleic acid materials, specifically into the genome of a host cell. The novel elements are derived from or based on transposons, in particular from the Tc/Mariner superfamily. In particular, the essential elements of Tc1 enabling excision and pasting of the desired nucleic acid material are provided, together with the relevant transposase activity in cis or in trans.
REFERENCES:
patent: 4686184 (1987-08-01), Puhler et al.
patent: 4830965 (1989-05-01), Narang et al.
patent: 5348874 (1994-09-01), Savakis et al.
patent: 5840865 (1998-11-01), Savakis et al.
#Bainton et al., "Tn7 Transposition In Vitro Proceeds through an Excised Transposon Intermediate Generated by Staggered Breaks in DNA," Cell, vol. 65, pp. 805-816, May 31, 1991.
#Bainton et al., "Tn7 Transposition: Target DNA Recognition Is Mediated by Multiple Tn7-Encoded Proteins in a Purified In Vitro System," Cell, vol. 72, pp. 931-943, Mar. 26, 1993.
#Beall et al., "A Drosophila protein homologous to the human p70 Ku autoimmune antigen interacts with the P transposable element inverted repeats," Proc. Natl. Acad. Sci. USA, vol. 91, pp. 12681-12685, Dec. 1994.
#Bender et al., "Genetic Evidence That Tn 10 Transposes by a Nonreplicative Mechanism," Cell, vol. 45, pp. 801-815, Jun. 20, 1986.
#Bolland et al., "The two single-strand cleavages at each end of Tn10 occur in a specific order during transposition," Proc. Natl. Acad. Sci. USA, vol. 92, pp. 7814-7818, Aug. 1995.
#Boyd et al., "The pCLIP plasmids: versatile cloning vectors based on the bacteriophage .lambda. origin of replication," Gene, 153, pp. 57-62, 1995.
#Clark, James M., "Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases," Nucleic Acids Research, vol. 16, No. 20, pp. 9677-9686, 1988.
#Collins et al., "Activation of a transposable element in the germ line but not the soma of Caenorhabditis elegans," Nature, vol. 328, 20, pp. 726-728, Aug. 1987.
Colloms et al., "DNA binding activities of the Caenorhabditis", Nucleic Acids Research, vol. 22, No. 25, pp. 5548-5554, 1994.
#Craxton, Molly, Linear Amplification Sequencing, a Powerful Method for Sequecing DNA, METHODS: A Companion to Methods in Enzymology, vol. 3, No. 1, pp. 20-26, Aug. 1991.
#Doak et al., "A proposed superfamily of transposase genes: Transposon-like elements in ciliated protozoa and a common "D35E" motif," Proc Natl. Acad. Sci. USA, vol. 91, pp. 942-946, Feb. 1994.
#Emmons et al., "Evidence for a Transposon in Caenorhabditis elgans," Cell, vol. 32, pp. 55-65, Jan. 1983.
#Fallaux et al., "Characterization of 911: A New Helper Cell Line for the Titration and Propagation of Early Region 1-Deleted Adenoviral Vectors," Human Gene Therapy, vol. 7 pp. 215-222, Jan. 20, 1996.
#Flinn et al., "Use of Gene Replacement to Construct Escherichia coli Strains Carrying Mutations in Two Genes Required for Stability of Multicopy Plasmids," Journal of Bacteriology, vol. 171, No. 4, pp. 2241-2243, Apr. 1989.
#Grindley et al., "Sequence Analysis at IS1 Insertion Sites: Models for Transposition," ENDFIELD Cold Spring Harbor Symp. Quant. Biol., 45, pp. 1257-1261.
#Grosschedl et al., HMG domain proteins: architectural elements in the assembly of nucleoprotein structures, TIG, vol. 10, No. 3, pp. 94-100, Mar. 1994.
Gunzburg et al., "Virus vector design in gene therapy", Molecular Medicine Today, vol. 1(9), pp. 410-417, 1995.
#Henikoff, Steven, "Detention of Caenorhabditis Transposon Homologs in Diverse Organisms," The New Biologist, vol. 4, No. 4, pp. 382-388, Apr. 1992.
#Kaufman et al., "P Element Transposition In Vitro Proceeds by a Cut-and-Paste Mechanism and Uses GTP as a Cofactor," Cell, vol. 69, pp. 27-39, Apr. 3, 1992.
#Kramer et al., "The Caenorhabditis elegans rol-6 Gene, Which Interacts with the sqt-1 Collagen Gene To Determine Organismal Morphology, Encodes a Collagen," Molecular and Cellular Biology, vol. 10., No. 5, pp.2081-2089, May 1990.
#Loukeris et al., "Gene Transfer into the Medfly, Ceratitis Capitata, with a Drosophila hydei Transposable Element," Science, vol. 270, pp. 2002-2005, Dec. 22, 1995.
#Mello et al., "Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences," The EMBO Journal, vol. 10, No. 12, pp. 3959-3970, 1991.
#Mizuuchi, Kiyoshi, "Transpositional Recombination: Mechanistic Insights from Studies of Mu and Other Elements," Annu. Rev. Biochem., vol. 61, pp. 1011-1051, 1992.
#Muller et al., "DNA bending creates favored sites for retroviral integration: an explanation for perferred insertion sites in nucleosomes," The EMBO Journal, vol. 13, No. 19, pp. 4704-4714, 1994.
#Nagai et al., "Synthesis and Sequence-Specific Proteolysis of Hybrid Proteins Produced in Escherichia coli," Methods Enzymol., 153, pp. 461-481.
#Paull et al., "The nonspecific DNA-binding and -bending proteins HMG1 and HMG2 promote the assembly of complex nucleoprotein structures," Genes & Development, vol. 7, pp. 1521-1534, 1993.
#Plasterk, Ronald H.A., "The origin of footprints of the Tc1 transposon of Caenorhabditis elegans," The EMBO Journal, vol. 10, No. 7, pp. 1919-1925, 1991.
#Plasterk, Ronald H.A., "Transposable Elements," Current Topics in Microbiology and Immunology, vol. 204, pp. 125-143, 1996.
#Pryciak, Peter M., "Nucleosomes, DNA-Binding Proteins, and DNA Sequence Modulate Rettoviral Integration Target Site Selection," Cell, vol. 69, pp. 769-780. May 29, 1992.
#Radice et al., "Widespread occurrence of the Tc1 transposon family: Tc1 -like transposons from teleost fish," Mol Gen Genet, vol. 244, pp. 606-612, 1994.
#Rio et al., "Evidence for Drosophila P Element Transposase Activity in Mannalian Cells and Yeast," J. Mol. Biol., vol. 200, pp. 411-415, 1988.
#Robertson, Hugh M., "The Tc1 -mariner Superfamily of Transposons in Animals," J. Insect Physiol., vol. 41, No. 2, pp. 99-105, 1995.
#Robertson, Hugh M., "The mariner transposable element is widespread in insects," Natur, vol. 362, pp. 241-245, Mar. 18, 1993.
#Robertson et al., "Recent Horizontal Transfer of a mariner Transposable Element among and between Diptera and Neuroptera," Mol. Biol. Evol., vol. 12(5), pp. 850-862, 1995.
#Schukkink et al., "TcA, the putative transposase of the C. elegans Tc1 transposon, has an N-terminal DNA binding domain," Nucleic Acids Research, vol. 18, No. 4, pp. 895-900. 1990.
#Shapiro, James a., "Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements," Proc. Natl. Acad, Sci. USA, vol. 76, No. 4, pp. 1933-1937, Apr. 1979.
#van Luenen et al., "Target site choice of the related transposable elements Tc1 and Tc3 of Caenorhabditis elegans," Nucleic Acids Research, vol. 22, No. 3, pp. 262-269, 1994.
#Vos et al., "Characterization of the Caenorhabditis elegans Tc1 transposase in vivo and in vitro," Genes & Development, vol. 7, pp. 1244-1253, 1993.
#Vos et al., "Tc1 transposase of Caenorhabditis elegans is a endonuclease with a bipartite DNA binding domain," The EMBO Journal, vol. 13, No. 24, pp. 6125-6132, 1994.
Vos et al., "Transposase is the only nematode protein required for in vitro transposition of Tc1", Genes & Development, 10:755-761 (1996).
#Way et al., "The mec-3 gene contains cis-acting elements mediating positive and negative regulation in cells produced by asymmetric cell division in Caenorhabditis elegans," Genes & Development, vol. 5, pp. 2199-2211, 1991.
#Zabarovsky et al., "High efficiency electroporation of ligated DNA into bacteria," Nucleic Acids Research, vol. 18, No. 19, p. 5912, 1990.
Plasterk Ronald Hans Anton
Schouten Govert Johan
Valerio Domenico
van Luenen Hendricus Gerhard Adrianus Maria
Vos Jan C.
Brusca John S.
Het Nederlands Kanker Instituut
IntroGene B.V.
Sandals William
LandOfFree
Vectors and methods for providing cells with additional nucleic does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Vectors and methods for providing cells with additional nucleic , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Vectors and methods for providing cells with additional nucleic will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2335559