Vectors and methods for making such vectors and for expressive c

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435 68, 4352523, 43525233, 4351721, 435320, 935 39, 935 40, 935 41, 935 44, 935 45, 536 27, C12N 1500, C12N 120, C12P 2100

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048747025

ABSTRACT:
Improved vectors and methods for expressing cloned genes of prokaryotic or eukaryotic origin and methods of making such vectors are disclosed, the improved vectors comprising promoters and operators from .lambda. phages and preferably do not include an active cro gene or an active N gene, the vectors having at least one endonuclease recognition site for cloning desired genes less than about 300 base pairs from the promoters and operators and being useful, as are methods utilizing the vectors, in producing a wide variety of prokaryotic, eukaryotic and viral polypeptides, hormones, enzymes, antigens, proteins and amino acids.

REFERENCES:
patent: 4237224 (1980-12-01), Cohen
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