Vectors and methods for making such vectors and for expressing c

Details Vectors and methods for making such vectors and for expressing c Vectors and methods for making such vectors and for expressing c

1991-09-03

1995-03-28

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4353201, 935 39, 935 40, 935 41, 935 44, 935 45, C12N 1500, C12N 1573

Patent

active

054016584

ABSTRACT:
Improved vectors and methods for expressing cloned genes of prokaryotic or eukaryotic origin and methods of making such vectors are disclosed, the improved vectors comprising promoters and operators from .lambda. phages and preferably do not include an active cro gene or an active N gene, the vectors having at least one endonuclease recognition site for cloning desired genes less than about 300 base pairs from the promoters and operators and being useful, as are methods utilizing the vectors, in producing a wide variety of prokaryotic, eukaryotic and vital polypeptides, hormones, enzymes, antigens, proteins and amino acids.

REFERENCES:
patent: 4874702 (1989-10-01), Fiers et al.

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