Vectors and methods for making such vectors and for expressing c

Details Vectors and methods for making such vectors and for expressing c Vectors and methods for making such vectors and for expressing c

1993-05-07

1995-03-28

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 695, 435 6951, 435 6952, 435 696, 4353201, C12P 2100, C12P 2102

Patent

active

054016428

ABSTRACT:
Improved vectors and methods for expressing cloned genes of prokaryotic or eukaryotic origin and methods of making such vectors are disclosed, the improved vectors comprising promoters and operators from .lambda. phages and preferably do not include an active cro gene or an active N gene, the vectors having at least one endonuclease recognition site for cloning desired genes less than about 300 base pairs from the promoters and operators and being useful, as are methods utilizing the vectors, in producing a wide variety of prokaryotic, eukaryotic and vital polypeptides, hormones, enzymes, antigens, proteins and amino acids.

REFERENCES:
Roberts et al; Proc. Natl. Acad. Sci. USA 76: 760 (1979).

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