Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Reexamination Certificate
2004-12-21
2009-12-08
Fronda, Christian L (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
C435S069100, C435S320100, C536S023100
Reexamination Certificate
active
07629160
ABSTRACT:
It is the object of the current invention to provide methods and compositions relating to the expression of vankyrin proteins in cell lines to increase their viability, longevity and capacity for protein production. The inventors have discovered that the expression of P-ank-1 and I2-ank-3 proteins in cell culture has increased the cells' longevity and capacity for endogenous and/or heterologous target protein production. Specifically, the present invention relates to the enhanced expression of endogenous and/or heterologous target proteins/polypeptides in recombinant cells that are also expressing P-ank-1 and/or I2-ank-3 protein compared to expression host cells that are not expressing P-ank-1 and/or I2-ank-3 protein.
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Kroemer Jeremy
Webb Bruce Allen
Fronda Christian L
McDermott Will & Emery LLP
University of Kentucky Research Foundation
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