Vectors and methods for enhanced cell longevity and protein...

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Reexamination Certificate

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C435S069100, C435S320100, C536S023100

Reexamination Certificate

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07629160

ABSTRACT:
It is the object of the current invention to provide methods and compositions relating to the expression of vankyrin proteins in cell lines to increase their viability, longevity and capacity for protein production. The inventors have discovered that the expression of P-ank-1 and I2-ank-3 proteins in cell culture has increased the cells' longevity and capacity for endogenous and/or heterologous target protein production. Specifically, the present invention relates to the enhanced expression of endogenous and/or heterologous target proteins/polypeptides in recombinant cells that are also expressing P-ank-1 and/or I2-ank-3 protein compared to expression host cells that are not expressing P-ank-1 and/or I2-ank-3 protein.

REFERENCES:
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Kroemer. J.A., and Webb, B.A. 2004. Brisbane, Queensland, Australia, submitted Mar. 31, 2004.
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