Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1987-02-02
1990-09-25
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 691, 435 711, 435171, 4351721, 435254, 435320, 435935, 530 27, 935 6, 935 9, 935 22, 935 23, 935 27, 935 52, 935 55, 935 56, 935 59, 935 60, 935 66, 935 68, C12N 113, C12N 1501, C12N 1552, C12N 1511
Patent
active
049593277
ABSTRACT:
Disclosed is a method for transforming Penicillium chrysogenum. More particularly, the method includes obtaining an auxotrophic mutant of P. chrysogenum and employing an exogeneous segment of P. chrysogenum DNA capable of complementing said auxotorph so as to restore prototrophy. The exogeneous DNA segment thus comprises a phenotypic marker indicating successful transformation of the mutant.
The exogeneous complementary DNA segment may further be prepared in a recombinant plasmid vector. These plasmid vectors include, by way of example, the pPctrpCL and pPctrpC.sub.6 plasmids developed by Applicants. These transforming plasmid vectors and those having identifying characteristics thereof are suitable for use in the subject transformation process. The exogeneous complementary DNA segment comprises a gene encoding a selected biosynthetic enzyme. By way of example, these genes include trpC, pyr4, argB and NO.sup.- reductase, as well as other genes which encode metabolically required enzymes. The most preferred of these is trpC.
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Ortega Agustin P. A.
Sanchez Florentina S.
Soto Miguel A. P.
Susan Victor R.
Antibiotics, S.A.
Peet Richard C.
Schwartz Richard A.
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