Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1984-09-27
1988-08-09
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 68, 435 70, 435253, 435254, 435320, 435886, 435925, 435926, 935 27, 935 28, 935 29, 935 56, 935 68, 935 75, C12N 1500, C12N 120, C12N 114, C12P 2100
Patent
active
047627867
ABSTRACT:
A method for transforming Cephalosporium and other lower eukaryotes is disclosed. The method involves inserting a recombinant DNA cloning vector comprising a Saccharomyces cerevisiae transcriptional and translational activating sequence positioned for expression of hygromycin phosphotransferase into a host cell and then growing the host cell under selective conditions. The vectors optionally further comprise Cephalosporium ribosomal DNA and also sequences that allow for replication and selection in E. coli and Streptomyces.
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Chapman, Jr. Jerry L.
Ingolia Thomas D.
Kaster Kevin R.
Queener Stephen W.
Skatrud Paul L.
Dahling Gerald V.
Eli Lilly and Company
Mays Thomas D.
Whitaker Leroy
Wiseman Thomas G.
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