Vector plasmid suited for souring agents, dairy souring agents i

Details Vector plasmid suited for souring agents, dairy souring agents i Vector plasmid suited for souring agents, dairy souring agents i

1987-06-24

1990-12-11

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 691, 435 711, 435 712, 4351723, 43525232, 43525233, 43525235, 435320, 935 29, 935 72, 935 73, 935 74, 935 75, C12N 120, C12N 1500, C12P 2100

Patent

active

049770888

ABSTRACT:
The invention relates to a cloning vector suited for the souring bacteria of dairy industries, which cloning vector is based on a 1.7 kb ClaI fragment of the Streptococcus lactis cryptic plasmid pSH71. Therein are inserted a chloramphenicol resistance gene from the Streptococcus sanguis plasmid pGB301 and an erythromycin resistance gene from the Staphylococcus aureus plasmid pE194, a 0.35 kb fragment from the Escherichia coli plasmis pBR322 being inserted therebetween. The size of the cloning vector is 5.0 kb. The cloning vector enables the use of "forced cloning" methods. It possesses a wide host spectrum (group N streptococci, Escherichia coli, Bacillus subtilis), and can be applied for improving the souring properties of the group N streptococci and for the preparation of foreign proteins in these bacteria or other hosts.

REFERENCES:
Gasson, et al. (1985) Fems Microbiology Letters, 30:193-196.
Kok et al; Appl. Environ. Microbiol. 48: 726 (1984).
Commercial Biotechnology: An International Analysis (Washington, D.C.: U.S. Congress, Office of Technology Assessment, OTA-BA-218, Jan. 1984).
Wallace et al: Science 209: 1396 (1980).
McKay (1983) Antonie van Leeuwenhoek 49:259-274.
Behnke et al., Mol. Gen Genet (1981) 184:115-120.
Weisblum, et al., J. Bacteriol (1979) 137:635-643.
Gasson, J. Bacteriol (1983) 154:1-9.
Kok, et al., (1985) Appl. Environ. Microbiol 50:94-101.

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