Vector mediated organelle transfection

Details Vector mediated organelle transfection Vector mediated organelle transfection

2002-12-12

2010-06-22

Falk, Anne-Marie (Department: 1632)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C435S320100

Reexamination Certificate

active

07741112

ABSTRACT:
The present invention provides compositions and methods for direct transfection of mitochondria and chloroplast DNA in living cells. More particularly, the present invention is based on the use of viral vectors that specifically bind to receptors uniquely found on the target organelle. In one embodiment, as shown inFIG. 1, a eukaryotic cell containing an organelle (1) that has been modified to express a viral receptor (2) on the organelle's surface is provided. A viral vector (6) comprising a desired recombinant DNA construct (3) is introduced into the cytosol of the cell, wherein the viral vector binds to its receptor and introduces the recombinant DNA into the interior of the organelle.

REFERENCES:
patent: 5831020 (1998-11-01), Citovsky
patent: 5854414 (1998-12-01), Hillman et al.
patent: 2007/0011759 (2007-01-01), Khan
patent: 2272788 (2000-12-01), None
patent: WO 98/46271 (1998-10-01), None
patent: WO 00/19993 (2000-04-01), None
Shore et al, (Eur J Biochem, 227: 9-18, 1995.
Ghosh et al, (Gene Expr, 7(3): 1-2, 1998.
Owen et al, (Human Gene Therapy, 11: 2067-2078, 2000.
de Grey et al, (TIBTECH, 18: 394-399, 2000).
Tommassen et al, (Mol Gen Genet , 197: 503-508, 1984).
D'Souza, G. , et al., “Gene Therapy of the other Genome : The Challenges of treating Mitochondrial DNA Defects”,Pharm Res.,24(2), Epub Dec. 19, 2006,(Feb. 2007),228-38.
Khan, S. M., et al., “Development of mitochondrial gene Replacement Therapy”,J Bioenerg Biomembr.,36(4), XP019280561,(Aug. 2004),387-93.
Maliga, P. , et al., “Plant biotechnology 2007: all three genomes make contributions to progress”,Current Opinion in Biotechnology,18(2), (Apr. 17, 2007),97-99.
Wallace, D. C., et al., “The mitochondrial genome in human adaptive radiation and disease: on the road to therapeutics and performance enhancement.”,Gene,354, XP005019916,(Jul. 18, 2005),169-80.

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