Vector having promoter that is inducible by methanol and/or glyc

Details Vector having promoter that is inducible by methanol and/or glyc Vector having promoter that is inducible by methanol and/or glyc

1995-06-07

1998-05-12

Vogel, Nancy T.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435192, 435194, 4353201, 4352542, 536 231, 536 232, 536 237, 536 241, C12P 2106, C12N 119, C12N 1581, C12N 1511

Patent

active

057503729

ABSTRACT:
The present invention relates to an expression vector which is induced by methanol and/or glycerol utilizing an alcohol oxidase gene of a methylotrophic yeast; a recombinant methylotrophic yeast containing said vector and capable of accumulating a significantly high amount of an expression product of a heterologous gene; and a method for producing useful products utilizing said recombinant.
The expression vector is constructed by utilizing the promoter and terminator of an alcohol oxidase gene of methylotrophic yeast. Further, a significantly high amount of adenylate kinase, cytochrome C552 or peroxidase is produced by using said expression vector.

REFERENCES:
Sreekrishna et al., High Level Expression of Heterologous Proteins in Methylotrophic Yeast Pichia Pastoris, J. Basis Microbiol., vol. 28, No. 4, 1988, pp. 265-278.
Sakai et al., Cloning and Sequencing of the Alcohol Oxidase-encoding Gene . . . , Gene, vol. 114, 22 Jan. 1992, Amsterdam, pp. 67-73.

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