Vector for the expression of fusion proteins and protein immunog

Details Vector for the expression of fusion proteins and protein immunog Vector for the expression of fusion proteins and protein immunog

1988-01-15

1990-11-27

Teskin, Robin L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 711, 435 91, 4351721, 4351723, 43525233, 435320, 536 27, 530350, 935 10, 935 29, 935 45, 935 47, 935 56, 935 73, C12P 2106, C12P 2104, C12P 1934, C12N 1500, C12N 120, C12H 1512, C07K 300

Patent

active

049735519

ABSTRACT:
An expression vector which can be used to express fusion proteins which are useful as immunogens. The vector is characterized as a 3.35 kilobase pair vector having origins for replication and selectivity markers for bacteria. The plasmid has an E. coli promotor segment, a CheY fusion protein sequence and a unique restriction site at the 3' end of the CheY segment for preparing a DNA segment which codes for a foreign protein to be expressed.

REFERENCES:
patent: 4639372 (1987-01-01), Murray et al.
patent: 4650676 (1987-03-01), Schenkel et al.
patent: 4710377 (1987-12-01), Schenkel et al.
Aviv and Leder, Proc. Natl. Acad. Sci. U.S.A. 69: 1408-1412 (1972).
Bethell et al. J. Biol. Chem. 254: 2572-2574 (1979).
Bhanushali and Long, in "Research in Avian Coccidiosis," McDougald et al. eds., Proceedings of Georgia Coccidiosis Conf., pp. 526.gtoreq.534 (1985).
Chirgwin et al., Biochem. 18: 5294-5299 (1979).
Clarke et al., Mol. and Biochem. Parasit, 22: 79-87 (1987).
Corthier et al., J. Immunol. Meth. 66: 75-79 (1984).
Crane et al., Infect. Immun. 56: 972-976 (1988).
Davis et al., Basic Methods in Molecular Biology, Elsievier, New York, N.Y. p. 30 (1986).
Edgar, Trans. Am. Micr. Soc. 62: 237-242 (1954).
Gubler and Hoffman, Gene 25: 263-269 (1983).
Hall et al., Nature 311: 379-382 (1984).
Hattori and Sakaki, Anal. Biochem. 152: 232-238 (1986).
Huynh et al., In "DNA Cloning: a Practical Approach," vol. I, Glover Ed., IRL Press, Oxford, pp. 49-78 (1985).
Ish-Horowicz and Burke, Nucleic Acids Research 9: 2989-2998 (1981).
Jackson, Parasitol. 54: 87-93 (1964).
Kohler and Milstein, Nature 256: 495-497 (1975).
Laemmli, Nature 227: 680-684 (1970).
Long et al., Biol. Abst. 75, No. 3, p. 1904, Ref. 18594 (1982).
Lowry et al., J. Biol. Chem. 193: 265-275 (1951).
MacPherson, Soft Agar Techniques, in "Tissue Culture Methods and Applications", Academic Press, pp. 276-277 (1973).
Maniatis et al., Molecular Cloning, a Laboratory Manual, Cold Spring Harbor Lab., pp. 202-203 and 468-469 (1982).
Mason and Williams, in "Nucleic Acid Hybridization: a Practical Approach" Hames and Higgens, Eds., IRL Press, pp. 113-137 (1985).
Matsumura et al., J. Bactol. 160: 36-41 (1984).
Maxam and Gilbert, Methods in Enzymology 65: (part 1): 499-559 (1980).
Messing, Methods in Enzymology 101: 20-78 (1983).
O'Farrell, J. Biol. Chem. 250: 4007-4021 (1975).
Okayama and Berg, Mole. Cell. Biol. 2: 161-170 (1982).
Old and Primrose, Principles of Gene Manipulation: University of California Press, p. 20 (1981).
Patton, Science 150: 767-769 (1965).
Patton et al., J. Parasito. 5: 526-530 (1979).
Rose and Kesketh, Parasitol. 73: 25-37 (1976).
Santoro et al., J. Biol. Chem. 258: 3341-3345 (1983).
Schmatz et al., J. Protozool. 31: 181-183 (1984).
Schneider et al., J. Biol. Chem. 257: 10766-10769 (1982).
Smithies et al., Science 202: 1284-1289 (1978).
Tabor and Richardson, Proc. Natl. Acad. Sci. U.S.A. 84: 4767-4771 (1987).
Taylor et al., Mol. Biochem. Parasitol. 10: 305-318 (1983).
Temeles et al., Nature 313: 700-703 (1985).
Towbin et al., Proc. Natl. Acad. Sci. U.S.A. 76: 4350-4354 (1979).
Weir, Handbook of Experimental Immunology Blackwell Scientific Publ. London, pp. A3.11-A3.15 (1978).
Wisher, Mol. Biochem. Parasitol. 21: 7-15 (1986).
Yanisch-Perron et al., Gene 33:103-119 (1985).
James, Parasitol. 80: 301-312 (1980).
Johnson and Reid, Exp. Parasitol. 28: 30-36 (1970).

Affiliated with

Also associated with

Rating

Vector for the expression of fusion proteins and protein immunog does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Vector for the expression of fusion proteins and protein immunog, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Vector for the expression of fusion proteins and protein immunog will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1031719