Vector for the cloning and expression of .gamma.-interferon, tra

Details Vector for the cloning and expression of .gamma.-interferon, tra Vector for the cloning and expression of .gamma.-interferon, tra

1989-06-02

1992-01-14

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4352523, 43525233, 4353201, 536 27, 935 6, 935 38, 935 45, 935 47, 935 73, C12N 1570, C12N 1511, C12N 1500, C12P 2102

Patent

active

050810227

ABSTRACT:
The present invention relates to a vector for the expression of the protein of human .gamma.interferon in bacteria, of the type containing the gene which codes for the protein of human .gamma.interferon and the plasmid elements which provide for the expression of this gene, wherein the 5' end of the sequence coding for the protein is as follows: ##STR1## The bacteria transformed by these vectors enable .gamma.-IFN to be produced in high yield.

REFERENCES:
patent: 4578355 (1986-03-01), Rosenberg
patent: 4582800 (1986-04-01), Crowl
patent: 4695623 (1987-09-01), Stabinsky
patent: 4762791 (1988-08-01), Goeddel et al.
Gray et al; Nature 295: 503 (1982).
Gene, vol. 13, 1981, Elsevier/North-Holland Biomedical Press; A. Honigman et al: "Plasmid vectors for positive selection of DNA inserts controlled by the lambda rho L promoter, repressor and antitermination function", pp. 289-298.

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