Vector for in vitro mutagenesis and use thereof

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification

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4353201, 435471, 435 6, 435 691, 435 4, 435 29, 435 34, 43525233, 435 914, 435 681, 4352523, 536 231, 536 234, 536 243, 536 235, C12K 1500

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059553634

ABSTRACT:
Site-directed in vitro mutagenesis is obtained utilizing a specially-engineered plasmid vector. The vector is engineered to contain an inactivated genetic marker which marker is capable of being reverted to functional expression. Additionally, the vector contains a second genetic marker, a polylinker region and an f1 replication origin.

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