Vector for high level gene expression

Details Vector for high level gene expression Vector for high level gene expression

1984-11-16

1989-01-17

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 70, 435 91, 4351723, 435317, 435320, 935 6, 935 22, 935 29, 935 48, 935 73, 536 27, C12P 2100, C12P 1934, C12N 500, C12N 700

Patent

active

047987910

ABSTRACT:
Methods and vectors for high level expression of genes in bacteria are disclosed. A terminal mRNA sequence from a gene coding for a stable bacterial protein mRNA is ligated to a gene coding for the desired protein adjacent the translation termination codon of the gene. The gene for the desired protein and the terminal mRNA sequence are situated in an expression vector in which the gene is operably linked to a transcription promoter.

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