Vector for expression of polypeptides in bacilli

Details Vector for expression of polypeptides in bacilli Vector for expression of polypeptides in bacilli

1985-03-29

1989-01-31

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

4351723, 435221, 435320, 435839, 43525231, 935 29, 935 48, 935 74, C12P 2100, C12N 1500, C12N 954, C12N 120

Patent

active

048015377

ABSTRACT:
A replicable plasmidic expression vector, capable of high levels of expression and secretion of polypeptides in a bacillus is disclosed. The vector contains a DNA sequence comprising the promoter and regulatory regions which control expression and secretion of proteases in a bacillus operably liked to a DNA sequence encoding the amino acid sequence of a polypeptide. The expression vector is particularly useful in the production of B. amyloliquefaciens proteases or other heterologous proteins in B. subtilis.

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