Vector for expression and secretion of polypeptide microorganism

Details Vector for expression and secretion of polypeptide microorganism Vector for expression and secretion of polypeptide microorganism

1995-02-24

1996-08-13

Elliott, George C.

Chemistry: molecular biology and microbiology

Vector, per se

536 234, 536 2351, C12N 1570, C12N 1562, C12N 1518

Patent

active

055455646

ABSTRACT:
This present invention provides a vector for expression and secretion of a polypeptide by directly joining a DNA sequence coding for a signal peptide to a DNA sequence coding for the desired polypeptide, the vector containing the DNA sequence coding for the signal peptide, a vector for expression and secretion of the desired polypeptide as defined above and further having incorporated therein the DNA sequence coding for the desired polypeptide, a microorganism transformed by the secretion vector, and a process for producing the desired polypeptide by growing the microorganism.

REFERENCES:
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Moreno et al., "A Signal Sequence is Not Sufficient to Lead .beta.-Galactosidase Out of the Cytoplasm", Nature, vol. 286, pp. 356-359, Jul. 24, 1980.
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