Vector constructs for the selection and identification of open r

Details Vector constructs for the selection and identification of open r Vector constructs for the selection and identification of open r

1997-03-13

1999-11-09

Degen, Nancy

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 691, 4351723, 4353201, 536 231, C12Q 168, C07H 2104, C12N 1563, C12P 2102

Patent

active

059811820

ABSTRACT:
The present invention provides for novel vector constructs comprising an origin of replication; a nucleotide sequence encoding an intein, the nucleotide sequence having a unique restriction enzyme site, critical amino acid residues located at the splice junctions of the intein, the intein inserted into a nucleotide sequence encoding a selectable marker; and a nucleotide sequence encoding suitable regulatory elements so as to effect expression of the vector construct in a suitable host cell. The vector constructs of the present invention may contain a DNA of interest cloned into a unique restriction site of the intein, and may be used as a vaccine alone or transformed into a vaccine vector. The vector constructs of this invention may further be used in methods of selecting translated open reading frames or genes, leading to the identification of potentially protective antigens of pathogenic organisms.

REFERENCES:
patent: 5496714 (1996-03-01), Comb et al.
Smith et al., "Multiplex Sequencing of 1.5 Mb of the Mycobacterium Ieprae Genome", Genome Research, 7: 802-819, 1997.
Norton, Microbiology, Second Ed., Addison Wesley Publishing Company, Inc., pp. 654-687, 1986.
Haynes, Science, 260: 1279-1286, 1993.
Barton F. Haynes, entitled "Scientific and Social Issues of Human Immunodeficiency Virus Vaccine Development," Science, vol. 260, pp. 1279-1286 May (1993).
Cynthia Friend Norton, entitled "Circulatory Infections," Microbiology, Chapter 22, pp. 654-687.
Douglas R. Smith, et al., entitled "Multiplex Sequencing of 1.5 Mb of the Mycobacterium leprae Genome," Genome Research, pp. 802-819, 1997.

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