Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1994-04-05
1997-09-09
Fleisher, Mindy
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4353201, 435325, 435354, 435355, C12N 510, C12N 1563, C12N 1564
Patent
active
056655789
ABSTRACT:
Disclosed are vectors for achieving high level expression in eucaryotic cells. The vectors include an expressible gene encoding a protein product of interest, an expressible gene encoding a marker protein which permits selection of useful transformants, and an enhancer element, preferably a cellular enhancer element, which functions to increase the level of transcription of genes disposed on its 3' and 5' sides. A blocking element is interposed between the enhancer element and the marker gene which shields the promoter of the marker gene from the transcription-stimulating function of the enhancer, thereby limiting the effect of the enhancer to transcriptions of the DNA encoding the protein product of interest. Use of the vectors permits isolation of viable clones characterized by a very high level of expression of the protein of interest.
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Fleisher Mindy
McKelvey Terry A.
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