Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Hormone or other secreted growth regulatory factor,...
Reexamination Certificate
2000-10-30
2003-05-27
Eyler, Yvonne (Department: 1646)
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Hormone or other secreted growth regulatory factor,...
C530S350000, C435S069400, C514S002600
Reexamination Certificate
active
06569434
ABSTRACT:
BACKGROUND OF THE INVENTION
A new class of cell-derived dimeric mitogens with apparently restricted specificity for vascular endothelial cells has recently been identified and generally designated vascular endothelial growth factors (VEGFs). The mitogen has been purified from: conditioned growth media of rat glioma cells, [Conn et al., Proc. Nat. Acad. Sci. USA 87: 1323-1327 (1990)]; conditioned growth media of bovine pituitary folliculo stellate cells [Ferrara and Henzel, Biochem. Biophys. Res. Comm. 161: 851-858 (1989) and Gospodarowicz et al., Proc. Natl. Acad. Sci. USA 86: 7311-7315 (1989)]. An endothelial cell growth factor isolated form mouse neuroblastoma cell line NB41 with an unreduced molecular mass of 43-51 kDa and a reduced mass of 23-29 kDa has been described by Levy et al., Growth Factors 2: 9-19 (1989). Connolly et al. (J. Biol. Chem. 264: 20017-20024 [1989]; J. Clin. Invest. 84: 1470-1478 [1989]) describe a human vascular permeability factor that stimulates vascular endothelial cells to divide in vitro and promotes the growth of new blood vessels when administered into healing rabbit bone grafts or rat corneas. An endothelial cell growth factor has been purified from the conditioned medium of the AtT-20 pituitary cell line by Plouet et al., EMBO Journal 8: 3801-3806 (1989). The growth factor was characterized as a heterodimer composed of subunits with molecular mass of 23 kDa. Leung et al. (Science 246: 1306-1309 [1989]), Keck et al. (Science 246: 1309-1312 [1989]) and Conn et al. (Proc. Natl. Acad. Sci USA 87: 2628-2632 [1990]) have described cDNAs which encode VEGF A which is homologous to the A and B chains of platelet-derived growth factor. Vascular endothelial growth factor I (VEGF I, VEGF AA) is a homodimer with an apparent molecular mass of 46 kDa, with each subunit having an apparent molecular mass of 23 kDa. VEGF I has distinct structural similarities to platelet-derived growth factor (PDGF), a mitogen for connective tissue cells but not vascular endothelial cells from large vessels.
OBJECTS OF THE INVENTION
It is, accordingly, an object of the present invention to provide novel vascular endothelial growth factor C subunit DNA free of other mammalian DNA. Another object is to provide recombinant genes capable of expressing VEGF C subunit or dimer. Another object is to provide vectors containing the DNA sequences for VEGF A or B plus C subunits. A further object is to provide a host cell transformed with a vector containing the DNA sequence for VEGF A or B plus C or VEGF C alone. It is also an object to provide a recombinant process for making VEGF C subunit. Another object is to provide a novel vascular endothelial cell growth factor which contains the C subunit. This may include heterodimers AC and BC and homodimer CC.
SUMMARY OF THE INVENTION
Vascular endothelial cell growth factor C subunit DNA is prepared by polymerase chain reaction techniques. The DNA encodes a protein that may exist as either a heterodimer or homodimer. The protein is a mammalian vascular endothelial cell mitogen and as such is useful for the promotion of vascular development and repair. This unique growth factor is also useful in the promotion of tissue repair.
REFERENCES:
patent: 5219739 (1993-06-01), Tischer et al.
patent: 5332671 (1994-07-01), Ferrara et al.
patent: 5607918 (1997-03-01), Eriksson et al.
patent: 6361946 (2002-03-01), Alitalo et al.
patent: 0 259 953 (1988-03-01), None
patent: 0 370 989 (1990-05-01), None
patent: 0 399 816 (1990-11-01), None
patent: 0 476 983 (1992-03-01), None
patent: 90/13649 (1990-11-01), None
patent: 91/02058 (1991-02-01), None
Conn et al. “Amino Acid and cDNA sequences of a vascular endothelial cell mitrogen that is homologous to platelet-derived growth factor”; Proc. Natl. Acad. Sci., vol. 87, pp 2628-32 (1990).
Conn et al. “Purification of aglycoprotein vascular endothelial cell mitogen from a rat glioma-derived cell line”; Proc. natl. Acad. Sci., vol. 87, pp 1323-17 (1990).
Ferrara, et al. “Pituitary Follicular Cells secrete a novel Heparin-Binding Growth Factor Specific for Vascualr Endothelial Cells”; Biochem. Biophyus. Res. Comm., vol. 161, pp 851-8 (1989).
Levy, et al. “An Endothelial Cell Growth Factor from the Mouse Neuroblastoma Cell Line NB41”; Growth Factors, pp 9-19, vol. 2 pp 9-19 (1989).
Saiki et al. “Enzymatic Amplification of Beta-Globoin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia”; Science, vol. 230, pp 1350-4 (1985).
Plouet et al. “Isolation and Characterization of a newly identified endothelial cell mitogen produced by A1T-20 cells”; EMBO Journal, vol. 8, pp 3801-6 (1989).
Connolly et al. “Human Vascular Permeability Factor”; J. Biol. Chem. vol. 264, pp 20017-24 (1989).
Laemmli et al. “Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4”; Nature, vol. 227, pp 680-5 (1970).
Frohman et al. “Rapid production of full length cDNAs from a rare transcripts; Amplification using a single gene-specific oligonucleotide primer”; Proc. Natl. Acad. Sci., vol. 85, pp 8998-9002 (1988).
Gospodarowicz et al. “Isolation and characterization of a vascular endothelial cell mitogen produced by pituitary-derived folliculo stellate cells”; Proc. Natl. Acad. Sci., vol. 86, pp 7311-5 (1989).
Keck et al. “Vascular Permeability Factor, an Endothelial Cell Mitogen Related to PDGF”; Science, vol. 246, pp 1309-12 (1989).
Leung et al. “Vascular Endothelial Growth Factor is a Secreted Angiogenic Mitogen”; Science, vol. 246, pp 1306-9 (1989).
Connolly e tal. “Tumor Vascular Permeability Factor Stimulates Endothelial Cell Growth and Angiogensis”; J. Clin. Inves., vol. 84, pp 1470-8 (1989).
Conn, G.L. “Endothelial Cell Growth Factors”; Yeshiva University, N. Y. 1987).
Maglione et al. ‘Isolation of a human placenta cDNA . . . permealbility factor’; Proc. Natl. Acad. Sci. USA vol. 88, pp 9267-9271, Oct. 1991.
Bayne Marvin L.
Thomas, Jr. Kenneth A.
Andres Janet L
Eyler Yvonne
Hand J. Mark
Merck & Co. , Inc.
Tribble Jack L.
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