Variety classification method for barley or malt using gene diag

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 911, 435 912, 536 231, 536 243, 536 2433, 800235, 935 76, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2104, C12N 1500

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058076760

ABSTRACT:
The variety of barley or malt is quickly and conveniently classified by amplifying the genomic DNA by PCR with the primer consisting of the sequence complementary to the gene that is important for brewing and examining the difference in the base sequence of said DNA.

REFERENCES:
Yoshigi et al., "PCR Cloning and Sequencing of the beta-Amylase cDNA from Barley," J. Biochem. 115, 47-51, Jan. 1994.
Forde, B.G., et al., Nucleic Acids Research vol. 13, No. 20: 7327-7339, 1985.
S. Weining et al., Theo. Appl. Genet. 82: 209-216, 1991.
N. Yoshigi et al., J. Biochem. 115: 47-51, 1994.
R.J. Rahmatullah et al., Plant Mol. Biol. 12: 119-121, 1989.
J.C. Litts et al., Eur. J. Biochem. 194: 831-838, 1990.
P.W. Chee et al. "Development of Polymerase Chain Reaction for Barley Genome Analysis", Journal Am.Soc.Brewing Chemists, (1993), vol. 1, No. 3, pp. 93-96.

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