Variants of the α1 subunit of human AMPK

Details Variants of the α1 subunit of human AMPK Variants of the α1 subunit of human AMPK

2007-04-24

2007-04-24

Achutamurthy, Ponnathapura (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

C435S006120, C435S069100, C435S320100, C536S023100, C536S023200

Reexamination Certificate

active

10503038

ABSTRACT:
Variants of the α subunit of AMP-activated kinase (AMPK), nucleic acids encoding such variants, and methods for their use are provided. The AMPK variants include splice variants that have an additional exon in the AMPK mRNA, and thus have an additional 15 amino acid residues in the encoded polypeptide. The AMPK variants can, for example, have at least 75% identity to the amino acid sequence of SEQ ID NO:4, and can contain an amino acid sequence having at least 75% identity to the amino acid sequence of SEQ ID NO:2.

REFERENCES:
patent: 4946778 (1990-08-01), Ladner et al.
patent: 5846720 (1998-12-01), Foulkes et al.
patent: WO 97/25341 (1997-07-01), None
Ota et al. (GenBank Accession No. AK024252, created Sep. 30, 2000)Homo sapienscDNA FLJ14190 fis, clone NT2RP2006354, moderately similar to 5′-AMP-activated protein kinase, catalytic alpha-1 chain (EC 2.7.1.-).
Verhoeven et al. “The AMP-activated protein kinase gene is highly expressed in rat skeletal muscle: Alternative splicing and tissue distribution of the mRNA”, Eur. J. Biochem. 228: 236-243, 1995.
GenBank Accession No. AB022017 dated Jan. 8, 1999, 2 pages.
GenBank Accession No. AAH48980 dated Apr. 22, 2003, 2 pages.
GenBank Accession No. NM—006251 dated Oct. 18, 2005, 8 pages.
Cheung et al., “Characterization of AMP-activated protein kinase γ-subunit isoforms and their role in AMP binding,”Biochem. J., 2000, 346:659-669.
Cole et al.,Monoclonal Antibodies and Cancer Therapy; 1983, Alan R. Liss, Inc., pp. 77-96.
Collins, “Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences,”Proc. Natl. Acad. Sci. USA, 2002, 99(26):16899-16903.
Cote et al., “Generation of human monoclonal antibodies reactive with cellular antigens,”Proc. Natl. Acad. Sci. USA, 1983, 80:2026-2030.
Davies et al., “Tissue distribution of the AMP-activated protein kinase, and lack of activation by cyclic-AMP-dependent protein kinase, studied using a specific and sensitive peptide assay,”Eur. J. Biochem., 1989, 186:123-128.
Engh and Bossemeyer, “The Protein Kinase Activity Modulation Sites: Mechanisms for Cellular Regulation—Targets for Therapeutic Intervention,”Advan. Enzyme Regul., 2001, 41:121-149.
Hardie and Carling, “The AMP-activated protein kinase. Fuel gauge of the mammalian cell?”Eur. J. Biochem., 1997, 246:259-273.
Hardie et al., “The AMP-Activated/SNF1 Protein Kinase Subfamily: Metabolic Sensors of the Eukaryotic Cell?”Annu. Rev. Biochem., 1998, 67:821-855.
Hardie and Hawley, “AMP-activated protein kinase: the energy charge hypothesis revisited,”BioEssays, 2001, 23:1112-1119.
Holmes et al., “Chronic activation of 5′-AMP-activated protein kinase increases GLUT-4, hexokinase, and glycogen in muscle,”J. Appl. Physiol., 1999, 87(5):1990-1995.
Huse et al., “Generation of a Large Combinatorial Library of the Immunoglobulin Repertoire in Phage Lambda,”Science, 1989, 246:1275-1281.
International Human Genome Sequencing Consortium,Nature, 2001, 409:860-921.
Köhler and Milstein, “Continuous cultures of fused cells secreting antibody of predefined specificity,”Nature, 1975, 256:495-497.
Kozbor and Roder, “The production of monoclonal antibodies from human lymphocytes,”Immunology Today, 1983, 4(3):72-79.
Michell et al., “Isoform-specific Purification and Substrate Specificity of the 5′-AMP-activated Protein Kinase,”J. Biol. Chem., 1996, 271(45):28445-28450.
Winder and Hardie, “AMP-activated protein kinase, a metabolic master switch: possible roles in Type 2 diabetes,”Am. J. Physiol., 1999, 277:E1-E10.
Zhou et al., “Role of AMP-activated protein kinase in mechanism of metformin action,”J. Clin. Invest., 2001, 108(8):1167-1174.

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