Variants of glycoside hydrolases

Details Variants of glycoside hydrolases Variants of glycoside hydrolases

2011-04-26

2011-04-26

Prouty, Rebecca (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C510S382000, C435S267000

Reexamination Certificate

active

07932073

ABSTRACT:
The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

REFERENCES:
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Ståhlberg et al., 1996,J. Mol. Biol. 264: 337-349.
Harry Boer and Anu Koivula, 2003,Eur. J. Biochem. 270: 841-848.
Ståhlberg et al., 2001,J. Mol. Biol. 305: 79-93.
Becket et al., Engineering of a glycosidase Family 7 cellobiohydrolase to more alkaline pH optimum: the pH behaviour of Trichoderma reesei CeI7A and its E223S/A224H/L225V/T226A/D262G mutant, Biochem. J. (2001) 356, 19-30.
Wey et al., Molecular Cloning and Sequence Analysis of the Cellobiohydrolase I Gene from Trichoderma koningii G-39, Current Microbiology, vol. 28 (1994) pp. 31-39.

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