Variants of beta-glucosidases

Details Variants of beta-glucosidases Variants of beta-glucosidases

2004-04-30

2008-08-19

Prouty, Rebecca (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S069100, C435S006120, C435S252300, C435S320100, C536S023200

Reexamination Certificate

active

07413888

ABSTRACT:
The present invention relates to variants of a parent beta-glucosidase, comprising a substitution at one or more positions corresponding to positions 142, 183, 266, and 703 of amino acids 1 to 842 of SEQ ID NO: 2 or corresponding to positions 142, 183, 266, and 705 of amino acids 1 to 844 of SEQ ID NO: 70, wherein the variant has beta-glucosidase activity. The present invention also relates to nucleotide sequences encoding the variant beta-glucosidases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

REFERENCES:
patent: 0126586 (2001-05-01), None
Iwashita K et al. (GenBank Accession No. P87076, 1997).
Kawaguchi T et al. (GenBank Accession No. JC4939, 1996).
Shu et al. (GenBank Accession No. AAB82320, 2001).
Kawaguchi et al., Cloning and sequencing of the cDNA encoding Beta-glucosidase 1 fromAspergillus aculeatus, Gene, 1996, v. 173, pp. 287-288.
Iwashita et al., The bgIA gene ofAspergillus kawachiiencodes both extracellular and cell wall-bound Beta-glucosidases, Applied and Environmental Microbiology, Dec. 1999, pp. 5546-5553.

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