Variant-type carbohydrate hydrolase, variant gene of the enzyme

Details Variant-type carbohydrate hydrolase, variant gene of the enzyme Variant-type carbohydrate hydrolase, variant gene of the enzyme

1994-03-02

1996-07-23

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435202, 435203, 4351723, 43525233, 536 232, C12N 910, C12N 930, C12N 1554, C12N 1556

Patent

active

055388825

ABSTRACT:
There is disclosed a variant-type carbohydrate hydrolase that has been increased transglycosylation activity by substituting another amino acid residue for the tyrosine residue that is present in the active center of the hydrolase, which hydrolase is an amylase or an enzyme analogous to amylase; a gene or a DNA sequence of the carbohydrate hydrolase with mutation introduced into the base sequence that encodes the tyrosine residue; and a vector or a transformant which comprises the DNA sequence. There is also disclosed a method for producing a variety of oligosaccharides and the like by using the variant-type carbohydrate hydrolase.

REFERENCES:
Matsui, I. et al. Biochemistry 33(2): 451-458 (1994).
Nakamura, A. et al. Biochemistry 33(33): 9929-9936 (1994).
Matsui, I. et al. Biochemistry 31:5232-5236 (1992).

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