Variant proteins and polypeptides possessing enhanced affinity f

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Hormones – e.g. – prolactin – thymosin – growth factors – etc.

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Details

530350, 530413, 435 691, C07K 318, C07K 1500, C12N 1509

Patent

active

051151022

ABSTRACT:
The present invention is directed at variant proteins and polypeptides having an enhanced affinity, i.e., greater binding strength, for immobilized-metal affinity resins and resides in engineering one or more specific metal-chelating amino acid sequences into a protein or polypeptide, the specific sequence depending on the metal-binding amino acids utilized and the secondary structure associated with the portion of the protein or polypeptide to include such sequence.

REFERENCES:
patent: 4569794 (1986-02-01), Smith et al.
Scopes, R. K., 1987, Protein Purification: Principles and Practices, Springer Verlag, New York, pp. 234-235.
Seeburg et al., 1983, DNA 2(1):37-45.
Smith et al., J. Bio. Chem., 263 (15), 7211 (1988).
Affinity Chromatography and Related Techniques, Gribnau et al., Editors, Elsevier Scientific Publishing Co., Amsterdam (1982).
Sulkowski, Texas Rep. on Biol. and Med., 41, 234 (1981).
Hochuli et al., Biotechnology, 6, 1321 (1988).
Hochuli, J. Chrom., 444, 293 (1988).
Sulkowski, Trends in Biotechnology, 3, 1 (1985).
Hemdan et al., Proc. Natl. Acad. Sci., 86, 1811 (1989).

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