Variant lipid acyltransferases and methods of making

Details Variant lipid acyltransferases and methods of making Variant lipid acyltransferases and methods of making

2011-03-15

2011-03-15

Mondesi, Robert (Department: 1645)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

C435S007100, C435S018000, C435S183000, C435S196000, C530S350000

Reexamination Certificate

active

07906307

ABSTRACT:
The present invention relates to a method for the production of a variant lipid acyltransferase comprising the steps of: (i) selecting a parent enzyme which is a lipid acyltranserase enzyme; (ii) modifying one or more amino acids to produce a variant lipid acyltransferase; (iii) testing the activity of the variant lipid acyltransferase on a galactolipid and/or phospholipid and/or triglyceride substrate; (iv) selecting a variant enzyme with enhanced activity towards galactolipids compared with the parent enzyme; (v) providing aBacillus licheniformiscell; (vi) transforming theBacillus licheniformiscell with a heterologous nucleotide sequence encoding said variant lipid acyltransferase; and (iii) expressing said variant lipid acyltransferase in the cell under the control of a promoter sequence. The variant lipid acyltransferase can undergo post-translations modification, truncation and/or clipping, i.e., to remove a signal peptide. In addition, the present invention further relates to the use ofBacillus licheniformisto express a lipid acyltransferase, aBacillus licheniformishost cell comprising a heterologous lipid acyltransferase and a vector comprising a nucleotide sequence encoding a lipid acyltransferase operably linked to a promoter sequence homologous toB. licheniformis.

REFERENCES:
patent: 2888385 (1959-05-01), Grandel
patent: 3260606 (1966-07-01), Azuma
patent: 3368903 (1968-02-01), Johnson
patent: 3520702 (1970-07-01), Menzi
patent: 3634195 (1972-01-01), Melachouris
patent: 3652397 (1972-03-01), Pardun
patent: 3677902 (1972-07-01), Aunstrup
patent: 3852260 (1974-12-01), Knutsen
patent: 3973042 (1976-08-01), Kosikowski
patent: 4034124 (1977-07-01), Van Dam
patent: 4065580 (1977-12-01), Feldman
patent: 4160848 (1979-07-01), Vidal
patent: 4202941 (1980-05-01), Terada
patent: 4399218 (1983-08-01), Gauhl
patent: 4567046 (1986-01-01), Inoue
patent: 4683202 (1987-07-01), Mullis
patent: 4689297 (1987-08-01), Good
patent: 4707291 (1987-11-01), Thom
patent: 4707364 (1987-11-01), Barach
patent: 4708876 (1987-11-01), Yokoyama
patent: 4798793 (1989-01-01), Eigtved
patent: 4808417 (1989-02-01), Masuda
patent: 4810414 (1989-03-01), Huge-Jensen
patent: 4814331 (1989-03-01), Kerkenaar
patent: 4818695 (1989-04-01), Eigtved
patent: 4826767 (1989-05-01), Hansen
patent: 4865866 (1989-09-01), Moore
patent: 4904483 (1990-02-01), Christensen
patent: 4916064 (1990-04-01), Derez
patent: 5112624 (1992-05-01), Johna
patent: 5213968 (1993-05-01), Castle
patent: 5219733 (1993-06-01), Myojo
patent: 5219744 (1993-06-01), Kurashige
patent: 5232846 (1993-08-01), Takeda
patent: 5264367 (1993-11-01), Aalrust
patent: 5273898 (1993-12-01), Ishii
patent: 5288619 (1994-02-01), Brown
patent: 5290694 (1994-03-01), Nakanishi
patent: 5378623 (1995-01-01), Hattori
patent: 5523237 (1996-06-01), Budtz
patent: 5536661 (1996-07-01), Boel
patent: 5558781 (1996-09-01), Buchold
patent: 5650188 (1997-07-01), Gaubert
patent: 5677160 (1997-10-01), Oester
patent: 5695802 (1997-12-01), Van Den Ouweland
patent: 5763383 (1998-06-01), Hashida
patent: 5766912 (1998-06-01), Boel
patent: 5776741 (1998-07-01), Pedersen
patent: 5814501 (1998-09-01), Becker
patent: 5821102 (1998-10-01), Berka
patent: 5827719 (1998-10-01), Sandal
patent: 5830736 (1998-11-01), Oxenboll
patent: 5834280 (1998-11-01), Oxenboll
patent: 5856163 (1999-01-01), Hashida
patent: 5863759 (1999-01-01), Boel
patent: 5869438 (1999-02-01), Svendsen
patent: 5874558 (1999-02-01), Boel
patent: 5879920 (1999-03-01), Dale
patent: 5892013 (1999-04-01), Svendsen
patent: 5914306 (1999-06-01), Svendsen
patent: 5916619 (1999-06-01), Miyazaki
patent: 5919746 (1999-07-01), Hirayama
patent: 5929017 (1999-07-01), Gormsen
patent: 5965384 (1999-10-01), Boel
patent: 5965422 (1999-10-01), Loffler
patent: 5976855 (1999-11-01), Svendsen
patent: 5989599 (1999-11-01), Chmiel
patent: 5990069 (1999-11-01), Andre
patent: 6001586 (1999-12-01), Schellenberger
patent: 6001640 (1999-12-01), Loeffler
patent: 6020180 (2000-02-01), Svendsen
patent: 6066482 (2000-05-01), Steffens
patent: 6074863 (2000-06-01), Svendsen
patent: 6103505 (2000-08-01), Clausen
patent: 6110508 (2000-08-01), Olesen
patent: 6140094 (2000-10-01), Loffler
patent: 6143543 (2000-11-01), Michelsen
patent: 6143545 (2000-11-01), Clausen
patent: 6146869 (2000-11-01), Harris
patent: 6156548 (2000-12-01), Christensen et al.
patent: 6180406 (2001-01-01), Stemmer
patent: 6254645 (2001-07-01), Kellis
patent: 6344328 (2002-02-01), Short
patent: 6350604 (2002-02-01), Hirayama
patent: 6358543 (2002-03-01), Soe et al.
patent: 6361974 (2002-03-01), Short
patent: 6365204 (2002-04-01), Spendler
patent: 6432898 (2002-08-01), Rey
patent: 6495357 (2002-12-01), Fuglsang
patent: 6506588 (2003-01-01), Tsutsumi
patent: 6509182 (2003-01-01), Tsutsumi
patent: 6511837 (2003-01-01), Tsutsumi
patent: 6514739 (2003-02-01), Udagawa
patent: 6558715 (2003-05-01), Rey
patent: 6582942 (2003-06-01), Christensen
patent: 6624129 (2003-09-01), Borch
patent: 6645749 (2003-11-01), Vind
patent: 6682922 (2004-01-01), Berka
patent: 6686189 (2004-02-01), Rey
patent: 6726942 (2004-04-01), Søe et al.
patent: 6730346 (2004-05-01), Rey
patent: 6815190 (2004-11-01), Abo
patent: 6852346 (2005-02-01), Soe et al.
patent: 6936289 (2005-08-01), Olsen et al.
patent: 6967035 (2005-11-01), Bojsen et al.
patent: 7226771 (2007-06-01), Gramatikova et al.
patent: 2002/0098536 (2002-07-01), Norinobu
patent: 2002/0110854 (2002-08-01), Tsutsumi
patent: 2002/0142434 (2002-10-01), Tsutsumi
patent: 2002/0168746 (2002-11-01), Tsutsumi
patent: 2003/0003561 (2003-01-01), Vind
patent: 2003/0028923 (2003-02-01), Lardizabal
patent: 2003/0040450 (2003-02-01), Rey
patent: 2003/0074695 (2003-04-01), Farese
patent: 2003/0100092 (2003-05-01), Berka
patent: 2003/0119164 (2003-06-01), Udagawa
patent: 2003/0148495 (2003-08-01), Hastrup
patent: 2003/0180418 (2003-09-01), Rey
patent: 2003/0185939 (2003-10-01), Nielsen
patent: 2003/0215544 (2003-11-01), Nielsen
patent: 2004/0005399 (2004-01-01), Chakrabarti
patent: 2004/0235106 (2004-11-01), Kapeller-Libermann
patent: 2004/0235119 (2004-11-01), Hoppe et al.
patent: 2005/0059130 (2005-03-01), Bojsen
patent: 2005/0059131 (2005-03-01), Bisgard-Frantzen
patent: 2005/0118697 (2005-06-01), Budolfsen
patent: 2005/0142647 (2005-06-01), Wassell
patent: 331094 (1995-02-01), None
patent: 249546 (1996-12-01), None
patent: P000105426 (2000-10-01), None
patent: P040101441 (2004-04-01), None
patent: 110 768 (1987-08-01), None
patent: 570720 (1984-09-01), None
patent: 723031 (1998-04-01), None
patent: 199742798 (1998-04-01), None
patent: 754470 (1999-11-01), None
patent: 8404421-7 (1984-04-01), None
patent: 805618 (1969-02-01), None
patent: 462382 (1984-09-01), None
patent: 1270781 (1990-06-01), None
patent: 2012723 (1990-09-01), None
patent: 2134597 (1994-10-01), None
patent: 2224143 (1996-12-01), None
patent: 2 403 025 (2004-04-01), None
patent: 97181706.5 (1998-12-01), None
patent: 036151 (2002-02-01), None
patent: 172509 (2003-06-01), None
patent: 2817087 (1978-11-01), None
patent: 19620649 (1997-11-01), None
patent: 69129968 (1999-03-01), None
patent: 69330066 (2001-10-01), None
patent: 69527835 (2003-04-01), None
patent: 69528070 (2003-06-01), None
patent: 69333065 (2003-07-01), None
patent: 69904161 (2003-07-01), None
patent: 69716711 (2003-09-01), None
patent: 69531538 (2004-06-01), None
patent: 69819782 (2004-09-01), None
patent: 3106.200 (1989-01-01), None
patent: 157560 (1990-01-01), None
patent: PA0888/92 (1992-07-01), None
patent: 0217/94 (1994-02-01), None
patent: PA0830/95 (1995-07-01), None
patent: PA1096/95 (1995-09-01), None
patent: 152763 (1998-03-01), None
patent: PA0543/98 (1998-04-01), None
patent: PA199801572 (1998-11-01), None
patent: PA5677000 (1998-12-01), None
patent: PA199801604 (1998-12-01), None
patent: 5559.215 (1999-10-01), None
patent: PA199901736 (1999-12-01), None
patent: PA200000989 (2000-06-01), None
patent: PA200000991 (2000-06-01), None
patent: PA200100285 (2001-02-01), None
patent: PA200100843 (2001-05-01), None
patent: PA200300634 (2003-04-01), None
patent: 0064855 (1982-11-01), None
patent: 0010296 (1982-12-01), None
patent: 01092

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