Variant Humicola grisea CBH1.1

Details Variant Humicola grisea CBH1.1 Variant Humicola grisea CBH1.1

2004-03-26

2008-12-02

Saidha, Tekchand (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S069100, C435S105000, C435S320100, C536S023200, C510S320000

Reexamination Certificate

active

07459299

ABSTRACT:
Disclosed are variants ofHumicola griseaCel7A (CBH1.1),H. jecorinaCBH1 variant orS. thermophiliumCBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.

REFERENCES:
patent: 4822516 (1989-04-01), Suzuki et al.
patent: 5246853 (1993-09-01), Clarkson et al.
patent: 5475101 (1995-12-01), Ward et al.
patent: 6268328 (2001-07-01), Mitchinson et al.
patent: 2005/0277172 (2005-12-01), Day et al.
patent: 0137280 (1985-04-01), None
patent: WO 91/04673 (1991-04-01), None
patent: WO 92/06209 (1992-04-01), None
patent: WO 94/28117 (1994-12-01), None
patent: WO 98/21339 (1998-05-01), None
patent: WO 98/31821 (1998-07-01), None
Takashima et al. UniProt Accession No. Q12621, created Nov. 1, 1996, “Cloning, sequencing, and expression of the cellulase genes ofHumicola griseavar.thermoidea”.
Lange et al. WO2003000941, publication Jan. 3, 2003, filed on Jun. 26, 2002, “New polypeptide with cellobiohydrolase I activity, useful in producing ethanol from biomass”.
de Oliviera et al. Sequence of cbh-1 gene ofHumicola griseavar.thermoidea, Nucleic Acids Research, vol. 18, p. 668, 1990.
Aleksenko, Alexei et al., “The plasmid replicator AMA1 inAspergillus nidulansis an inverted duplication of a low-copy-number dispersed genomic repeat,”Molecular Microbiology, 19(3):565-574, 1996.
**Alexopoulos, C.J., (1962), Introductory Mycology, New York:Wiley.
Altschul, Stephen F. et al., “Basic Local Alignment Search Tool,”J. Mol. Biol., 215:403-410, 1990.
Altschul, Stephen F. et al., “Gapped Blast and PSI-Blast: a new generation of protein database search programs,”Nucl. Acids Res., vol. 25, pp. 3389-3402, 1997.
Aro, Nina et al., “ACEII, a Novel Transcriptional Activator Involved in Regulation of Cellulase and Xylanase Genes ofTrichoderma reesei,” J. Biol. Chem., 276(26):24309-24314, 2001.
**Aubert, et al., Ed., p. 11 et seq., Academic Press, 1988.
**Ausubel et al., Current Protocols in Molecular Biology, Greene Publishing, Associates and Wiley Interscience, N.Y. (1994).
Baker, John O. et al., “A New Thermostable Endoglucanase,Acidothermus cellulolyticusE1,”Applied Biochemistry and Biotechnology, 45/46:245-256, 1994.
Baker, John O. et al., “Hydrolysis of Cellulose Using Ternary Mixtures of Purified Cellulases,”Applied Biochemistry and Biotechnology, 70-72:395-403, 1998.
Ballance, D.J. et al., “Transformation ofAspergillus nidulansby the Orotidine-5′-Phosphate Decarboxylase Gene ofNeurospora crassa,” Biochemical and Biophysical Research Communications, 112(1):284-289, Apr. 15, 1983.
Barclay, Stephen L. et al., “Efficient Transformation ofDictyostellum discoideumAmoebae,”Molecular and Cellular Biology, 3:2117-2130, 1983.
Becker, Dieter et al., “Engineering of a glycosidase Family 7 cellobiohydrolase to more alkaline pH optimum: the pH behaviour ofTrichoderma reeseiCel7A and its E223S/A224H/L225V/T226A/D262G mutant,”Biochem. J., 356:19-30 (2001).
Berges, Thierry et al., “Isolation of uridine auxotrophs fromTrichoderma reeseiand efficient transformation with the clonedura3andura5genes,”Curr. Genet., 19:359-365, 1991.
Bhikhabhai, Ramagauri et al., “Isolation of Cellulolytic Enzymes fromTrichoderma reeseiQM 9414,”J. Appl. Biochem., 6:336-345, 1984.
Boel, E. et al., “Two different types of intervening sequences in the glucoamylase gene fromAspergillus niger,” The EMBO Journal, 3(7):1581-1585, 1984.
Brumbauer, Aniko et al., “Fractionation of cellulase and β-glucosidase in aTrichoderma reeseiculture liquid by use of two-phase partitioning,” Bioseparation, 7:287-295, 1999.
Campbell, Edward I., et al., “Improved transformation efficiency ofAspergillus nigerusing the homologousniaD gene for nitrate reductase,”Current Genetics, 16:53-56, 1989.
Cao, Qing-Na et al., “Penicillopepsin-JT2, a recombinant enzyme fromPenicillium janthinellumand the contribution of a hydrogen bond in subsite S3to kcat,”Protein Science, vol. 9, pp. 991-1001, 2000.
**Coligan, J. E. et al., eds., Current Protocols in Immunology, 1991.
Deutscher, Murray P., “Rethinking Your Purification Procedure,” Methods in Enzymology, vol. 182, No. 57, p. 779, 1990.
Ellouz, S. et al., “Analytical Separation ofTrichoderma reeseiCellulases by Ion-Exchange Fast Protein Liquid Chromatography,”Journal of Chromatography, 396:307-317, 1987.
Filho, Edivaldo X. F., “Purification and characterization of a β-glucosidase from solid-state cultures ofHumicola griseavar.thermoidea,” Can. J. Microbiol., 42:1-5, 1996.
Fliess, A. et al., “Characterization of Cellulases by HPLC Separation,”Eur. J. Appl. Microbiol. Biotechnol., 17:314-318, 1983.
**Freshney, R. I., ed.,Animal Cell Culture, 1987.
Gateway™ Cloning Technology, Intruction Manual, Version 1, pp. 34-38. (Date not given).
Gloss, Lisa M. et al., “Urea and Thermal Equilibrium Denaturation Studies on the Dimerization Domain ofEscherichia coli TrpRepressor,”Biochem., vol. 36, No. 19, pp. 5612-5623, 1997.
Goedegebuur, Frits et al., “Cloning and relational analysis of 15 novel fungal endoglucanases from family 12 glycosyl hydrolase,”Current Genetics, vol. 41, pp. 89-98, 2002.
Goyal, Anil, et al., “Characteristics of Fungal Cellulases,”Bioresource Technology, vol. 36, pp. 37-50, 1991.
**Hale & Markham, The Harper Collins Dictionary of Biology, Harper Perennial, NY, 1991.
Halldorsdottir, S, et al., “Cloning, sequencing and overexpression of aRhodothermus marinusgene encoding a thermostable cellulase of glycosyl hydrolase family 12,”Appl. Microbiol. Biotechnol., 49(3):277-284, 1998.
Hazell, B. W. et al., “Rapid transformation of high cellulase-producing mutant strains ofTrichoderma reeseiby Microprojectile bombardment,”Letters in Applied Microbiology, 30:282-286, 2000.
Herr, D. et al., “Purification and Properties of an Extracellular β-Glucosidase fromLenzites trabea,” European Appl. Microbiol. Biotechnol., 5:29-36, 1978.
Hu, Qianjin, et al., “Antibodies Specific for the Human Retinoblastoma Protein Identify a Family of Related Polypeptides,”Molecular and Cellular Biology, vol. 11, No. 11, pp. 5792-5799, 1991.
Hynes, Michael J., et al., “Isolation of Genomic Clones Containing theamdSGene ofAspergillus nidulansand Their Use in the Analysis of Structural and Regulatory Mutations,”Molecular and Cellular Biology, 3:1430-1439, 1983.
Ilmen, Marja et al., “Regulation of Cellulase Gene Expression in the Filamentous FungusTrichoderma reesei,” Appl. and Envir. Micro., vol. 63, No. 4, pp. 1298-1306, Apr. 1997.
Innis, M. A. et al., “Expression, Glycosylation, and Secretion of anAspergillusGlucoamylase bySaccharomyces cerevisiae,” Science, 228:21-26, 1985.
Jeenes, David J. et al., “Heterologous Protein Production by Filamentous Fungi,”Biotechnology and Genetic Engineering Reviews, vol. 9, pp. 327-367, 1991.
Kawaguchi, Takashi et al., “Cloning and sequencing of the cDNA encoding β-glucosidase 1 fromAspergillus aculeatus,” Gene, 173:287-288, 1996.
Kelly, Joan M. et al., “Transformation ofAspergillus nigerby theamdS gene ofAspergillus nidulans, ” The EMBO Journal, 4(2):475-479, 1985.
Knowles, Jonathan et al., “Cellulase families and their genes,”TIBTECH 5, pp. 255-261, 1987.
**Kriegler, Gene Transfer and Expression: A Laboratory Manual, 1990.
Krishna, S. Hari et al., “Simultaneous saccharification and fermentation of lignocellulosic wastes to ethanol using a thermotolerant yeast,”Bioresource Tech., 77:193-196, 2001.
Kuhls, K. et al., “Molecular evidence that the asexual industrialfungusTrichoderma reeseiis a clonal derivative of the ascomyceteHypocre

Affiliated with

Also associated with

Rating

Variant Humicola grisea CBH1.1 does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Variant Humicola grisea CBH1.1, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Variant Humicola grisea CBH1.1 will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4030592