Variant E1 protein gene for pyruvate dehydrogenase complex and v

Details Variant E1 protein gene for pyruvate dehydrogenase complex and v Variant E1 protein gene for pyruvate dehydrogenase complex and v

1994-03-22

1995-07-11

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435 691, 435189, 43525233, 4353201, 536 232, 935 10, 935 14, 935 29, 935 73, C12N 904, C12N 1553, C12N 1570

Patent

active

054320712

ABSTRACT:
The present invention relates to variant E.sub.1 protein of pyruvate dehydrogenase complex of high activity in which arginine at the 146-position is replaced by proline in the amino acid sequence of wild-type E.sub.1 protein of pyruvate dehydrogenase complex, a gene coding for said variant E.sub.1 protein, a novel recombinant DNA comprising said variant E.sub.1 protein-encoding gene inserted in a vector DNA, and a process for producing variant E.sub.1 protein by said recombinant DNA. The present invention provides the variant E.sub.1 protein of pyruvate dehydrogenase complex of high activity.

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