Variant DNA polymerases

Details Variant DNA polymerases Variant DNA polymerases

1995-06-06

1999-07-27

Houtteman, Scott W.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

935 77, 935 82, 935 11, C12N 900

Patent

active

059289198

ABSTRACT:
Variant family B DNA polymerases, examples of which have reduced or no 3'.fwdarw.5' exonuclease activity. These variant polymerases have utility as DNA sequencing polymerases.

REFERENCES:
Reha-Krantz et al., Proc. Natl. Acad. Sci., 88:2417-2421, Mar. 1991.
Reha-Krantz et al., J. Mol. Biol., 189(2):262-272, May 1986.
Reha-Krantz, L.J., et al., "Bacteriophage T4 DNA Polymerase Mutations That Confer Sensitivity to the PP; Analog Phosphonoacetic Acid", J. Virol., 67(1):60-66 (1993).

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