Variant aequorin genes and process for producing variant aequori

Details Variant aequorin genes and process for producing variant aequori Variant aequorin genes and process for producing variant aequori

1987-10-08

1992-03-03

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 91, 4351723, 4352351, 4352523, 4353201, 43525233, 536 21, 530350, C12P 2102, C12P 1934, C12N 1500, C12N 700, C12N 120, C07H 1512, B07K 300

Patent

active

050932403

ABSTRACT:
Various variants of photoprotein aequorin (pAQ440), useful for elucidating the mechanism of its luminescence and thereby extending the possibility of concrete applications of aequorin protein, and a process for producing variant aequorin proteins are provided, which variants are obtained by converting base(s) in a specified order of the base arrangement of aequorin gene into other base(s), or by deleting a certain bases in specified continued orders thereof, according to site-specific mutagenesis method.

REFERENCES:
Shimomura et al., Nature, vol. 256, pp. 236-238 (1975).
Tsuji, F. I., Proc. Natl. Acad. Sci. U.S.A., vol. 83, pp. 8107-8111 (1986).
Inouye et al., Proc. Natl. Acad. Sci. U.S.A., vol. 82, pp. 3154-3158 (1985).
Charbonneau et al., Biochem., vol. 24, pp. 6762-6771 (1985).
Itakura et al., Science, vol. 209, pp. 1401-1405 (1980).

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