Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Reexamination Certificate
2006-09-26
2006-09-26
Ponnaluri, Padmashri (Department: 1639)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
C530S387300, C435S005000, C435S320100, C435S328000, C435S334000, C435S091500, C435S091500, C424S130100, C424S133100, C424S178100
Reexamination Certificate
active
07112661
ABSTRACT:
The present invention is directed to a method of selecting a clone that binds to human platelet glycoprotein Ib alpha using a human variable heavy chain and variable light chain immunoglobulin library. The invention is further directed to isolated nucleic acid molecules encoding a variable heavy chain or variable light chain region of an antibody, wherein the antibody binds to human platelet glycoprotein Ib alpha and inhibits aggregation of platelets. Expression vectors and host cells comprising the nucleic acid molecules are also provided, as well as methods for producing the variable heavy chain or the variable light chain region. An isolated variable heavy chain or variable light chain region of an antibody, wherein the antibody binds to human platelet glycoprotein Ib alpha and inhibits aggregation of platelets, is also provided. An antibody comprising the variable heavy chain or variable light chain regions is provided, as is a composition comprising the antibody and a carrier. The subject invention further provides a method of inhibiting aggregation of platelets, as well as a method of binding human platelet glycoprotein Ib alpha. A method of selecting a variable heavy chain or variable light chain region of an antibody is also provided.
REFERENCES:
patent: 5486361 (1996-01-01), Gralnick
patent: 5817748 (1998-10-01), Miller et al.
patent: 5962255 (1999-10-01), Griffiths et al.
patent: 5969108 (1999-10-01), McCafferty et al.
patent: 2003/0077613 (2003-04-01), Soderlind et al.
patent: 0 372 670 (1990-06-01), None
patent: WO 92/14750 (1992-09-01), None
patent: WO 93/16712 (1993-09-01), None
patent: WO 96/17622 (1996-06-01), None
patent: WO 97/18236 (1997-05-01), None
patent: WO9832845 (1998-07-01), None
Okamoto et al (Tissue Antigens, vol. 51, pp. 156-163, 1998.
Miller et al (Arteriosclerosis and Thrombosis, vol. 11, No. 5, pp. 1231-1236, 1991).
Schroeder et al (Proc. Natl. Acad. Sci. USA. vol. 87. p. 6146-6150, Aug. 1990).
Raaphorst et al. (Eur. J. Immunol. 1992. vol. 22 . No. 1. pp. 247-251.
Carter, “Binders from the deepest vaults”, Nat Biotech 14(3):267 (1996).
Griffiths et al., “Isolation of high affinity human antibodies directly from large synthetic repertoires”, EMBO J 13(14):3245-3260 (1994).
Hiraiwa et al., “Sequence Analysis of Monoclonal Antibodies Derived From A Patient With Idiopathic Thrombocytopenic Purpura”, Autoimmunity 8:107-113 (1990).
Hoogenboom and Winter, “By-passing Immunisation. Human Antibodies from Synthetic Repertoires of Germline V(sub H) Gene Segments Rearranged in Vitro”, 227:381-388 (1992).
Konkle et al., “Cytokine-enhanced Expression of Glycoprotein Ib alpha in Human Endothelium”, J Biol Chem 265(32):19833-19838 (1990).
McCafferty et al., “Phage antibodies: filamentous phage displaying antibody variable domains”, Nature 348:552-554 (1990).
Miller and Lyle, “Mimotope / anti-mimotope probing of structural relationship in platelet glycoprotein Ib alpha”, Proc Natl Acad Sci USA 93:3565-3569 (1996).
Miller et al., “Selection of phagemid recognizing human platelet GPIb from a human V(sub H) and V(sub L) Immunoglobulin phagemid library”, Blood 90(10), Abstract 3016 (Nov. 15, 1997).
Miller et al., “Isolation and Characterization of Single Chain Human Antibodies Directed Against Epitopes Within Human Platelet GPIb alpha”, Blood 92(10), Abstract (Nov. 15, 1998).
Nissim et al., “Antibody fragments from a ‘single pot’ phage display library as immunochemical reagents”, EMBO J 13(3):692-698 (1994).
Sait, “The Human Synthetic VH+VL scFv Library / Agreement relating to the supply of The Human Synthetic VH+VL scFv Library / Protocol for use of the Human Synthetic VH+VL scFv Library”, Medical Research Council Center for Protein Engineering website (http://ind1.mrc-lmb.cam.ac.uk/cpe-home-page.html)(1997).
Tomlinson, “V Base. Introduction / V Base. Sequence Alignments”, Medical Research Council Center for Protein Engineering website (http://ind1.mrc-lmb.cam.ac.uk/cpe-home-page.html)(1997).
Tomlinson et al., “The Repertoire of Human Germline V(sub H) Sequences Reveals about Fifty Groups of V(sub H) Segments with Different Hypervariable Loops”, J Mol Biol 227:776-798 (1992).
Vaughan et al., “Human Antibodies with Sub-nanomolar Affinities Isolated from a Large Non-immunized Phage Display Library”, Nature Biotech 14:309-314 (1996).
Ware et al., J. Biol. Chem., 268(11):8376-8382(1993).
Ponnaluri Padmashri
Rogalskyj & Weyand LLP
The Research Foundation of State University of New York
LandOfFree
Variable heavy chain and variable light chain regions of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Variable heavy chain and variable light chain regions of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Variable heavy chain and variable light chain regions of... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3534015