Chemistry: analytical and immunological testing – Involving immune complex formed in liquid phase
Reexamination Certificate
2005-04-08
2010-08-31
Helms, Larry R. (Department: 1643)
Chemistry: analytical and immunological testing
Involving immune complex formed in liquid phase
C436S518000, C530S387300, C435S069600
Reexamination Certificate
active
07785903
ABSTRACT:
The invention provides polypeptides comprising a variant heavy chain variable framework domain (VFR). In some embodiments, the amino acids defining the VFR form a loop of an antigen binding pocket. In an embodiment, the polypeptide is a variable domain of a monobody and has a variant VFR. The polypeptide may optionally comprise one or more complementary determining regions (CDRs) of antibody variable domains. In an embodiment, the polypeptide is a variable domain of a monobody and has a variant VFR and one or more variant CDRs. Libraries of polypeptides that include a plurality of different antibody variable domains generated by creating diversity in a VFR, and optionally, one or more CDRs are provided and may be used as a source for identifying novel antigen binding polypeptides that can be used therapeutically or as reagents. The invention also provides fusion polypeptides, compositions, and methods for generating and using the polypeptides and libraries.
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Bond Christopher J.
Sidhu Sachdev
Genentech Inc.
Gussow Anne M.
Helms Larry R.
Jones Day
LandOfFree
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