Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-09-06
1996-12-03
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 436 94, 436173, 250282, 250423P, 935 77, 935 78, C12Q 168, C12P 1934, G01N 3348, B01D 5944
Patent
active
055807330
ABSTRACT:
Method for analyzing a nucleic acid molecule, without fragmenting the molecule, by vaporizing a mixture of the molecule and a matrix by illuminating the mixture with visible laser light absorbed by the matrix and not by the nucleic acid molecule. The method is useful for determining the nucleotide sequence of a polynucleotide by using mass spectrometry to determine the molecular weights of individual single-stranded nucleic acid molecules in a population including a plurality of single-stranded nucleic acid molecules generated from the polynucleotide, each molecule having a different molecular weight, and one defined terminus and one variable terminus which terminates at a specific nucleotide.
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Levis Robert J.
Romano Louis J.
Wayne State University
Zitomer Stephanie W.
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