Vacuum injection capillary electrophoresis

Details Vacuum injection capillary electrophoresis Vacuum injection capillary electrophoresis

1989-05-01

1994-07-05

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, G01N 2726, G01N 27447

Patent

active

053264452

ABSTRACT:
In accordance with the present invention, a compliant pressure differential is applied across a separation column while its inlet is in a sample solution so that sample solution is introduced into the column. The profile of pressure differential over time is selected so that a desired volume of sample solution is introduced. Pressure and time are controlled with sufficient precision to yield repeatability of within 5%, and preferably within 1%, by volume. The pressure differential is sufficiently low and the time sufficiently long that an acceptable velocity profile can be achieved.

REFERENCES:
patent: 5207886 (1993-05-01), Lauer et al.
Rose, Jr. & Jorgenson, "Characterization and Automation of Sample Introduction Methods for Capillary Zone Electrophoresis" Analytical Chemistry, 60 (1988) pp. 642-648.
Verhaggen, P. E. M. et al "Simple Sampling Device for Capillary Isotachophoresis and Capillary Zone Electrophoresis" Journal of Chromatography, 452 (1988) pp. 615-622.

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