Vaccine for Pseudomonas aeruginosa

Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus

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424 88, 424 85, 424 87, 530395, 530402, A61K 3902

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active

046938915

ABSTRACT:
There is disclosed an immunizing composition comprising a non-toxic polysaccharide isolated from gram-negative bacteria, especially from Pseudomonas aeruginosa, covalently coupled to a non-toxic protein from the gram-positive bacterium Micrococcus luteus by means of a 4-12 carbon moiety. To prepare the above immunizing agent, the lipid A portion of lipopolysaccharide from a gram-negative bacterium is separated to give a non-toxic polysaccharide. Reactive aldehyde groups are generated on the non-toxic polysaccharide by selective oxidation. The non-toxic polysaccharide is then covalently coupled to a non-toxic protein isolated from said gram-positive bacterium, Micrococcus luteus, by means of a polyfunctional compound having 4-12 carbon atoms and having at least two functional groups which are reactive to the aldehyde groups on the non-toxic polysaccharide and to the carboxylic groups on the non-toxic protein.

REFERENCES:
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patent: 4578458 (1986-03-01), Pier
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Seid et al., Preparation and Characterization . . . Conjugates" JBC, 1981, pp. 7305-7310.
Abst. (Biosis) Verloes et al., Successful Immuno Therapy . . . Chemotherapy" Br J. Cancer 43(2), 1981, pp. 201-209.

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