Vaccine for Clostridium perfringens type E enterotoxemia of rabb

Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus

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A61K 3908

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042645883

ABSTRACT:
A vaccine for Clostridium perfringins type E enterotoxemia of rabbits is produced by harvesting (or centrifuging) an actively growing culture of the bacterium and subjecting the supernatant to a proteolytic enzyme to produce a proteinaceous toxin characterized by a molecular weight of from 70,000 to 80,000 daltons, and inactivating the toxin by addition of a fixing agent to produce the toxoid along with any other toxins that might be present.

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Hauschild, A. H. W. Clostridium-perfringens Toxins Types B C D+E Microbial Toxins vol. IIa Bacterial Protein Toxins XIXAcad. Press N.alpha.Y.alpha.1971:159-188.
Marasanova, L. P. ZhMikrobiol Epidemiol Immunobio 148 (8) 1971:116-121 Role of Clostridium-perfringens Types A B C D E+F in the Etiology of Gas Gangrene.
Sharmaeva, S. A. et al. ZhMikrobiol Epidemiol Immunobio 148 (8):58-63 1971, Study of Soluble Clostridium-perfringens Antigens Types D+E on Tissue Culture.
Zemlyanitskaya, E. P. et al. ZhMikrobiol Epidemiol Immunobio 146 (=):57-60 (1969), Effect of Proteolytic Enzymes on the Activity of Proto Toxins Clostridium perfringens Types D+E.
U2 Ermakova et al C.alpha.A.alpha.84:55085w (1976) Morphological Changes in the Cultures of Various Tissues Caused by Clostridium perfringens Toxins type B C D E+F.
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Orcutt, R. P. et al. (1978) Clostridium perfringens Type E Enterotoxemia as a cause of acute diarrheal death or "hemorrhagic typlitis" in rabbits Abtract 100 Publication 78-4 Amer. Assoc. Lab Anim. Sci.
Patton, N. M. et al.(1978) Enterotoxemia in Rabbits Lab Anim. Sci. 28:536-540.
Katsaras K Effect of Trypsin and the Method of Injection of the Immunogenic Action of Toxins of Clostridium perfringens Types D+E Vet. Bull. 145 (8) 4238 1975.
McDonel, J. L. et al. Histopathological Effect of Clostridium perfringens Enterotoxin in the Rabbit Ileum Vet. Bull. 46 (4) 1804 (1976).

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