Vaccine containing a campylobacter bacterium having an enhanced

Details Vaccine containing a campylobacter bacterium having an enhanced Vaccine containing a campylobacter bacterium having an enhanced

1997-05-30

1999-02-09

Naff, David M.

Drug, bio-affecting and body treating compositions

Nonspecific immunoeffector, per se ; or nonspecific...

Bacterium or component thereof or substance produced by said...

424 931, 424 934, 424802, 4352521, 435822, A01N 6300, C12N 120

Patent

active

058690662

ABSTRACT:
This invention relates generally to in vitro methods for inducing or enhancing expression of enteric bacterial antigens and/or virulence factors thereby producing antigenically enhanced enteric bacteria, to methods for using antigenically enhanced enteric bacteria and to vaccines comprising antigenically enhanced enteric bacteria. Further, a vaccine comprising a Campylobacter bacterium having en enhanced antigenic property is disclosed. The culture medium conditions comprise 0.05% to 3% bile or 0.025% to 0.6% of one or more bile acids or salts. In addition a divalent cation chelator can also be included in the culture medium. The bacteria culture is in a growth phase at early log phase and between early log phase and stationary phase. The enhanced antigenic property is a higher level of an immunogenic antigen when compared to the antigenic property of the same bacteria grown on brain heart infusion broth.

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