Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Fusion protein or fusion polypeptide
Patent
1995-05-30
1999-01-12
Caputa, Anthony C.
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Fusion protein or fusion polypeptide
42419611, 4241991, 4242101, 4242781, 4242801, 424816, 530396, 530402, 530412, 530416, 530417, 435 693, A61K 39145, A61K 39295, C07K 1411, C12N 1544
Patent
active
058583688
ABSTRACT:
A method of preparing a recombinant influenza vaccine using DNA technology is provided. The resulting vaccine is a multivalent, preferably trivalent, influenza vaccine based on a mixture of recombinant hemagglutinin antigens cloned from influenza viruses having epidemic potential. The recombinant hemagglutinin antigens are full length, uncleaved (HAO), glycoproteins produced from baculovirus expression vectors in cultured insect cells and purified under non-denaturing conditions. The recombinant vaccine can be developed from primary sources of influenza, for example, nasal secretions from infected individuals, rather than from virus adapted to and cultured in chicken eggs. The process for cloning influenza hemagglutinin genes from influenza A and B viruses uses specially designed oligonucleotide probes and PCR. In the preferred embodiment, the cloned HA genes are then modified by deletion of the natural hydrophobic signal peptide sequences and replacing them with a new baculovirus signal peptide.
REFERENCES:
patent: 4029763 (1977-06-01), Kilbourne
patent: 4289690 (1981-09-01), Pestka et al.
patent: 4659669 (1987-04-01), Kleid et al.
patent: 4752473 (1988-06-01), Nayak et al.
patent: 4920213 (1990-04-01), Dale et al.
patent: 5591823 (1997-01-01), Hung et al.
Smith et al. Mol. Cell Biology 3(12):2156-65, 1983.
Pennock et al. Mol. Cell Biology 4(3):399-406, 1984.
Kreuter, "Microcapsules and Nanoparticles in Medicine and Pharmacy," Ed. M. Dunbrow (1991).
Luckow et al., Bio/Technology, 6:47-55 (1988).
Stanley et al., Virology, 56:640-645 (1973).
Kuroda et al., EMBO Journal, 5(6): 1359-1365 (1986).
Hackett Craig S.
Smith Gale E.
Volvovitz Franklin
Voznesensky Andrei I.
Wilkinson Bethanie E.
Caputa Anthony C.
Kowalski Thomas J.
Protein Sciences Corporation
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