UV excitable fluorescent energy transfer dyes

Organic compounds -- part of the class 532-570 series – Organic compounds – Heterocyclic carbon compounds containing a hetero ring...

Reexamination Certificate

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C546S016000, C546S034000, C548S219000

Reexamination Certificate

active

07012141

ABSTRACT:
Novel energy transfer dyes which can be used with shorter wavelength light sources are provided. These dyes include a donor dye with an absorption maxima at a wavelength between about 250 to 450 nm and an acceptor dye which is capable of absorbing energy emitted from the donor dye. One of the energy transfer dyes has a donor dye which is a member of a class of dyes having a coumarin or pyrene ring structure and an acceptor dye which is capable of absorbing energy emitted from the donor dye, wherein the donor dye has an absorption maxima between about 250 and 450 nm and the acceptor dye has an emission maxima at a wave length greater than about 500 nm.

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Zlokarnik et al., 1998, “Quantitation of Transcription and Clonal Selection of Single Living Cells with β-Lactamase as Reporter,”Science279:84-88.

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