Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1990-05-24
1992-06-30
Fox, David T.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4352522, 435878, 424 941, 424 9461, 47 58, 71 7, C12N 502, C12R 141, A61K 3748, A61K 3754, A01H 100, C05F 1108
Patent
active
051262630
ABSTRACT:
Plant protoplasts are exposed for interaction with an external agent by exposing the plasma membrane of protoplasts in the root hairs of the plant without release of protoplasts from the plant. This may be achieved by enzymatically degrading the plant cell wall at the apices of root hairs under conditions which expose the plasma membrane whilst maintaining or readily permitting restoration of the essential functional integrity of the plant. Enzymatic degradation of the cell wall of the apices of root hairs is possible in a wide range of crop species and other species and to expose the plasma membrane with partial protoplast release. This enables direct interaction with plasmids, viruses and micro-organisms at the surface of the plasma membrane of their exposed protoplasts, or the fusion of these with isolated protoplasts of other plant systems.
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Fox David T.
University of Nottingham
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