Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-02-24
2008-09-23
Johannsen, Diana B (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S007920, C435S091200, C435S091520, C536S023700, C536S024320, C536S024330
Reexamination Certificate
active
07427483
ABSTRACT:
The present invention is the development of a simple and specific quantitative method for the determination ofP. falciparumDNA in malaria that involves the direct detection of the highly 42-kDa conserved C-terminal region ofP. falciparummerozoite surface protein (MSP1) gene. This procedure entails the amplification of the 42-kDa C-terminal region of the MSP1 gene by using the PCR technique in the presence of digoxigenin-11-dUTP and the synthesis of the specific biotin labeled nucleotide probes directed to the 42-kDa C-terminal region of the MSP1 gene. These specific probes are then used in the Enzyme Linked Immunosorbent Assay (ELISA) for the quantitative determination of the 42-kDa C-terminal region of the MSP1 gene which leads to the quantitative determination ofP. falciparumDNA in malaria for quantitative diagnostic purpose as well as for monitoring the efficacy of antimalarial treatment.
REFERENCES:
patent: 6855316 (2005-02-01), Chang et al.
Chang, S.P. et al. Experimental Parasitology 67:1-11 (1988).
Johannsen Diana B
Vista Biologicals Corporation
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